拮抗细菌C-02防治棉花黄萎病的机理研究

 从抗生、竞争和诱导系统抗性三个方面对拮抗细菌C-02防治棉花黄萎病的机理展开了研究，结果表明，C-02分泌的抗菌蛋白能溶解病原菌VD-11的细胞壁，抑制其致萎毒素的分泌。与病原菌VD-11相比，C-02利用碳源有明显优势，在棉花根际土壤中具有显著的营养竞争优势和很好的定殖能力。C-02处理棉苗后，棉叶中植株防御性酶系PAL、POD和PPO的活性提高幅度大，维持时间长。C-02防治棉花黄萎病的机理是抗生、溶菌、竞争和诱导棉苗系统抗性的综合表现。

Study on Antagonistic Mechanism of Bacterium Strain C-02 against Verticillium Wilt of Cotton

The mechanisms of antagonists against fungal pathogens have become increasingly important in biological control in recent decades. In this paper, the mechanisms of antagonism, competition, and systemic-resistance induction in the bacterium strain C-02 against Verticillium wilt of cotton were studied by double culture, liquid fermentation, and inducing cotton seedlings, respectively. The results indicated that an antifungal protein from strain C-02 could destroy the cell wall of pathogen strain VD-11 and inhibit its toxin secretion. The results also showed that strain C-02 could colonize the rhizospheric soil of cotton fields better than VD-11, and they had the advantage in carbon resource use. When cotton leaves were treated with a bacterial culture of C-02, PAL, POD and PPO activity was quickly enhanced and remained at high levels for a long time. In conclusion, the antagonistic mechanisms of C-02 against Verticillium wilt of cotton form a comprehensive behavior including antagonism, bacteriolysis, competition, and systemic-resistance induction.