| 大白菜地方种质种内遗传关系的RAPD分析 |
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| 引用本文： | 黄宝勇,曲士松,孙晋斌,张杰,徐培文,刘恒英. 大白菜地方种质种内遗传关系的RAPD分析[J]. 山东农业大学学报(自然科学版), 2002, 33(2): 154-157 |
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| 作者姓名： | 黄宝勇 曲士松 孙晋斌 张杰 徐培文 刘恒英 |
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| 作者单位： | 山东省农业科学院蔬菜研究所,济南,250100;山东省农业科学院蔬菜研究所,济南,250100;山东省农业科学院蔬菜研究所,济南,250100;山东省农业科学院蔬菜研究所,济南,250100;山东省农业科学院蔬菜研究所,济南,250100;山东省农业科学院蔬菜研究所,济南,250100 |
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| 摘 要： | 对大白菜亚种内11个地方种质材料,用改进的CTAB法提取DNA后进行RAPD（Random Amplified PolymorphieDNA)分析。聚类结果表明：当遗传距离为0．18时,11份材料可分为二个生态类群,第一类群属于直筒类型（交叉性气候生态型）,另一类群在0．15时,又分为二个类群,矮桩类型（海洋性气候生态型）和直筒与矮桩的过渡类型。同时表明,应用RAPD选择特定引物在同一亚种内不同品种间进行遗传多样性、亲缘关系及品种鉴定等研究是可行的。
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| 关 键 词： | 大白菜 遗传相似性 RAPD 聚类分析 |
| 文章编号： | 1000-2324(2002)02-0154-04 |
| 修稿时间： | 2001-04-28 |
STUDIES ON GENETIC RELATIONSHIPS AMONG LOCAL GERMPLASM OF CHINESE CABBAGE BY RAPD |
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| HUANG Bao-yong,QU Shi-song,SUN Jin-bin,ZHANG Jie,XU Pei-wen,LIU Heng-ying. STUDIES ON GENETIC RELATIONSHIPS AMONG LOCAL GERMPLASM OF CHINESE CABBAGE BY RAPD[J]. Journal of Shandong Agricultural University, 2002, 33(2): 154-157 |
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| Authors: | HUANG Bao-yong QU Shi-song SUN Jin-bin ZHANG Jie XU Pei-wen LIU Heng-ying |
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| Abstract: | RAPD was used to evaluate polymorphism and genetic relationship of 11 local germplasm of Chinese Cabbage.In the experiment 11 materials were divided into two ecological groups when genetic distance was 0.18,one was Crossed Climate Ecotype and the other was also divided into two groups when the distance was 0.15,one Ocean Climate Ecotype and the other was transitional form.Meanwhile,studies of genetic relationship and polymorphism by RAPD with specific primer within subspecies were feasible. |
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| Keywords: | Chinese cabbage genetic similarity RAPD cluster analysis |
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