| 鸡肝酯酶快速检测氰戊菊酯农药的试验研究 |
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| 引用本文： | 李思超,徐斐,赵洁,曹慧.鸡肝酯酶快速检测氰戊菊酯农药的试验研究[J].东北农业大学学报,2011,42(2):10-15. |
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| 作者姓名： | 李思超 徐斐 赵洁 曹慧 |
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| 作者单位： | 上海理工大学食品质量与安全研究所,上海,200093 |
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| 基金项目： | 上海市科委生物医药专项，上海市农委重点攻关项目 |
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| 摘 要： | 采用鸡肝酯酶对不同浓度的氰戊菊酯农药进行了催化水解,并通过pH-stat法优化了水解条件.结果表明,在pH 8.0,温度55℃的条件下,酶催化反应速度较大.在该反应条件下,分析了农药浓度(1/x)与酶解反应初速度(1/y)之间的关系,发现在0.05～2.00 mg·L-1农药浓度范围内,两者的关系曲线为y=0.0723...
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| 关 键 词： | 鸡肝酯酶 氰戊菊酯农药 pH-stat法 快速检测 |
Rapid determination of fenvalerate pesticide using chicken liver esterase |
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| LI Sichao,XU Fei,ZHAO Jie,CAO Hui.Rapid determination of fenvalerate pesticide using chicken liver esterase[J].Journal of Northeast Agricultural University,2011,42(2):10-15. |
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| Authors: | LI Sichao XU Fei ZHAO Jie CAO Hui |
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| Institution: | (Institute of Food Quality and Safety,University of Shanghai for Science and Technology,Shanghai 200093,China) |
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| Abstract: | Chicken liver esterase was used to catalyze the hydrolysis of fenvalerate and the optimal enzymatic reaction conditions were obtained as follows:pH 7.5 and temperature 55 ℃,using pH-state method. Based on the enzymatic hydrolysis of the fenvalerate reaction,the relationship between fenvalerate content(1/x) and initial velocity of enzymatic reaction(1/y) was discussed and the equation of y=0.0723x+0.1393(R2=0.998) was found as the standard curve for the calculation of fenvalerate content. And there was no significant difference between the results measured by the enzymatic method and GC method. The rapid method determination totally cost about 10 min,reducing a lot of test time than GC method. |
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| Keywords: | chicken liver esterase fenvalerate pesticide pH-stat rapid determination |
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