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| 偃麦草种质资源遗传多样性的RAPD分析 | |
| 引用本文： | 李培英,孙宗玖,朱昊,阿不来提. 偃麦草种质资源遗传多样性的RAPD分析[J]. 中国草地学报, 2012, 34(3): 12-20 |
| 作者姓名： | 李培英 孙宗玖 朱昊 阿不来提 |
| 作者单位： | 1. 新疆农业大学草业与环境科学学院/新疆草地资源与生态自治区重点实验室,新疆乌鲁木齐,830052 2. 新疆农业大学草业与环境科学学院/新疆草地资源与生态自治区重点实验室,新疆乌鲁木齐830052;新疆畜牧科学院草业研究所,新疆乌鲁木齐830000 |
| 基金项目： | 新疆农业大学草业科学重点学科，新疆高新技术课题，新疆高校科研计划 |
| 摘 要： | 通过对DNA提取方法及RAPD反应体系进行探讨,建立了适应偃麦草基因组DNA提取的方法及优化的RAPD反应体系。在此基础上,依据Jaccard相似系数,采用UPGMA方法进行聚类,判定32份偃麦草种质材料间的亲缘关系及遗传多样性。结果表明:(1)改良常规CTAB法,即对样品先进行洗涤并提高CTAB含量到3%可提高偃麦草DNA的提取质量。(2)对100条随机引物进行筛选,共筛选出18条多态性高、重复性好的随机引物,以此对32份材料进行PCR扩增,共扩增出264条带,多态性条带为93.7%。(3)以遗传相似系数为依据,对32份偃麦草材料进行聚类分析,在相似系数为0.373处可将供试偃麦草种质资源划分为5大类群。 |
| 关 键 词： | 偃麦草 新疆 RAPD 遗传多样性 |
Analysis of Genetic Diversity of 32 Elytrigia repens Accessions Based on RAPD Markers |
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| LI Pei-ying , SUN Zong-jiu , ZHU Hao , Abulaiti. Analysis of Genetic Diversity of 32 Elytrigia repens Accessions Based on RAPD Markers[J]. Chinese Journal of Grassland, 2012, 34(3): 12-20 | |
| Authors: | LI Pei-ying SUN Zong-jiu ZHU Hao Abulaiti |
| Affiliation: | LI Pei-ying1,SUN Zong-jiu1,ZHU Hao1,2,Abulaiti1 (1.College of Pratacultural and Environmental Science,Xinjiang Agricultural University / Key Laboratory of Grassland Resource and Ecology of Xinjiang,Urumqi 830052,China;2.Grassland Institute,Xinjiang Academy of Animal Science,Urumqi 830000,China) |
| Abstract: | Through exploring DNA extraction method and RAPD reaction system,the optimal experiment system was established.On this basis,analysis of genetic diversity of 32 Elytrigia repens accessions was carried out.Based on Jaccard similarity coefficient,cluster analysis was carried out with UPGMA method,genetic relationship and genetic diversity were determined.The results showed that(1)DNA of high quality could be obtained with improved conventional CTAB method namely first rinsing sample,increasing CTAB content(3%).(2) 264 bands of genome DNA of 32 accessions of E.repens were amplified with 18 effective random primers selected from 100 random primers,and the polymorphism rate was 93.7%.(3) By clustering analysis,at the point of genetic similarity coefficient 0.373,all materials could be divided into five groups. |
| Keywords: | Elytrigia repens Xinjiang RAPD Genetic diversity |
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