壳聚糖胁迫对黄独带芽茎段生长发育的影响

目的]研究壳聚糖胁迫对黄独带芽茎段生长发育的影响,以期为黄独的大田生产提供理论依据.方法]将黄独带芽茎段(带1个腋芽)接种至MS+KT 2 mg/L+NAA 0.5 mg/L的液体培养基中进行单因素4水平分组试验,壳聚糖浓度设0(CK)、0.5、1.0和2.0 g/L 4水平,定期进行观察并统计黄独试管苗的各种形态指标.结果]低浓度的壳聚糖对黄独带芽茎段根长和芽长有一定的促进作用,浓度为0.5 g/L时促进作用最明显;高浓度的壳聚糖抑制黄独带芽茎段根和芽的生长,浓度为1.0和2.0 g/L时抑制作用最明显.壳聚糖也抑制黄独带芽茎段叶片的生长,且浓度为1.0g/L时的抑制作用最明显.结论]壳聚糖胁迫对黄独带芽茎段生长发育的影响从总体上来说表现为低浓度促进高浓度抑制的特点.

Effects of chitosan on growth and development of Dioscorea bulbifeia L.

Objective] The effects of chitosan on growth and development of Dioscorea bulbifera L.stems was studied to facilitate its commercial production.Method]Single factor four-level group experiments were conducted,in which Dioscorea bulbifera L.stems having single bud were cultured in MS+KT(2mg/L)+NAA(0.5mg/L) culture medium.The concentration of chitosan was 0(CK),0.5,1.0 and 2.0 g/L.Various morphological indexes of Dioscorea bulbifeia L.plantlets were observed regularly and data was analyzed statistically.Result]Low concentration of chitosan enhanced the root and bud elongation to some extent in D.bulbifeia and the effect was most significant at 0.5 g/L concentration of chitosan.High concentration of chitosan inhibited the growth of root and bud in D bulbifeia stems.At 1.0-2.0 g/L concentrations,the effects were more pronounced.Chitosan also reduced the leaf growth of D bulbifeia plants,and the effects were more pronounced at its 1.0 g/L concentration.Conclusion]Low concentration of chitosan promoted the growth and development of D.bulbifeia stems,while high concentration of chitosan showed contrary results.