| 猪METTL3基因表达水平与DON诱导IPEC-J2细胞损伤的关系 |
| |
| 引用本文： | 许写,石东风,吴嘉韵,吴圣龙,王海飞,吴正常,包文斌.猪METTL3基因表达水平与DON诱导IPEC-J2细胞损伤的关系[J].畜牧兽医学报,2022,53(3):688-699. |
| |
| 作者姓名： | 许写 石东风 吴嘉韵 吴圣龙 王海飞 吴正常 包文斌 |
| |
| 作者单位： | 1. 扬州大学动物科学与技术学院 江苏省动物遗传繁育与分子设计重点实验室, 扬州 225009;2. 扬州大学 教育部农业与农产品安全国际合作联合实验室, 扬州 225009 |
| |
| 基金项目： | 江苏省农业科技自主创新资金项目(CX (20)3011);江苏省重点研发计划(现代农业)重点项目(BE2019344) |
| |
| 摘 要： | 旨在初步探究猪m6A甲基化酶METTL3基因表达水平与脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)诱导猪小肠上皮细胞(IPEC-J2)损伤的关系.本研究构建了稳定干扰METTL3基因表达水平的IPEC-J2细胞系,用1 μg·mL-1 DON诱导METTL3干扰组和对照组猪肠上皮细胞48 h,通过实时荧光...
|
| 关 键 词： | 猪 METTL3 脱氧雪腐镰刀菌烯醇 |
| 收稿时间： | 2021-07-26 |
Relationship between Expression Level of Porcine METTL3 Gene and the Injury of IPEC-J2 Cells Induced by DON |
| |
| XU Xie,SHI Dongfeng,WU Jiayun,WU Shenglong,WANG Haifei,WU Zhengchang,BAO Wenbin.Relationship between Expression Level of Porcine METTL3 Gene and the Injury of IPEC-J2 Cells Induced by DON[J].Acta Veterinaria et Zootechnica Sinica,2022,53(3):688-699. |
| |
| Authors: | XU Xie SHI Dongfeng WU Jiayun WU Shenglong WANG Haifei WU Zhengchang BAO Wenbin |
| |
| Institution: | 1. Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technoogy, Yangzhou University, Yangzhou 225009, China;2. Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China |
| |
| Abstract: | This study aimed to investigate the relationship between the expression level of porcine m6A methylases METTL3 and deoxynivalenol (DON) induced injury of intestinal porcine epithelial cells (IPEC-J2). In this study, IPEC-J2 cell lines with METTL3 knockdown was successfully established. IPEC-J2 cells from the METTL3 interference group and control group were treated with 1 μg·mL-1 DON for 48 h, respectively. Real-time quantitative PCR was used to detect the mRNA expression differences of apoptosis-related genes, immune-related genes and oxidase-related genes, and cell proliferation activity, cell cycle, cell apoptosis, and reactive oxygen species (ROS) levels were measured. The results showed that, compared with control group, the expression of immune-related genes TNF-α, IL-6 and IL-12 in the interference group changed significantly (P<0.05); The levels of apoptosis-related genes Bax, Caspase3 and Caspase9 were significantly decreased(P<0.05); The levels of oxidase-related genes GPXs, CuZn-SOD and CAT were significantly increased(P<0.05). The cell proliferation activity was significantly increased, and the total cell apoptosis rate was significantly decreased (P<0.05); The level of ROS was extremely significantly decreased (P<0.01). The results indicate that the down-regulation expression of METTL3 gene contributes to the enhancement of IPEC-J2 cells resisting apoptosis and inflammation induced by DON in a certain extent. The down-regulation expression of METTL3 gene is beneficial to alleviate a series of inflammatory responses and cell damage caused by DON, and will provide a theoretical basis for in-depth study of the mechanism of RNA methylation in the regulation of piglet resistance to DON damage in the future. |
| |
| Keywords: | pigs METTL3 deoxynivalenol |
|
| 点击此处可从《畜牧兽医学报》浏览原始摘要信息 |
| 点击此处可从《畜牧兽医学报》下载免费的PDF全文 |
|
