赛拉嗪-咪达唑仑复合麻醉剂对山羊大脑和海马NO/cGMP信号转导系统及神经递质的影响

旨在研究赛拉嗪复合咪达唑仑对山羊的麻醉效果,并检测中枢神经递质及NO/cGMP信号转导系统的变化,为山羊麻醉提供新的方法,并明确该复合制剂的全身麻醉作用机理.山羊肌内注射复合麻醉剂1.31 mL·kg-1,对照组注射生理盐水,观察动物的行为学变化,监测山羊的生理指标并评估麻醉效果,分别于麻醉诱导期、麻醉期、恢复Ⅰ期、恢...

Effects of Compound Anesthetics of Xylazine and Midazolam on NO/cGMP Signal Transduction System and Neurotransmitters in the Brain and Hippocampus of Goats

The goals of this study were to study the anesthetic effect of xylazine combined with midazolam on goats, and to detect the effects of combined anesthesia on central neurotransmitters and NO/cGMP signal transduction system, so as to provide a new method for goat anesthesia and to clarify the mechanism of general anesthesia of combined anesthesia. Goats received 1.31 mL·kg^―1 combined anesthetics with intramuscular injection, and the control group received normal saline. The behavioral changes of the animals were observed, and the physiological indexes and anesthesia effects were monitored. The contents of neurotransmitters and NO/cGMP pathway in the brain and hippocampus were detected by HPLC during induction, anesthesia, recovery I and recovery Ⅱ, respectively. Results were as follows: 1) xylazine-midazolam compound could induce anesthesia rapidly in goats, and the induction time was (5.63±2.35) min.Anesthesia maintenance time (56.72±5.38) min. The compound exhibited well sedation, analgesia and muscle relaxation effects, and has little effect on basic physiological parameters of goats; 2) During anesthesia, compound drugs inhibit the expression of norepinephrine (NE), dopamine (DA), aspartic acid (Asp) and glutamic acid (Glu) both in the brain and hippocampus, and increase the contents of serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA), glycine (Gly) in hippocampus and γ -aminobutyric acid (GABA) in the brain; 3) During the anesthetic period, the compound inhibited the expression of NO, NOS and cGMP in the brain and hippocampus. We can conclude that the xylazine-midazolam compound has a good anesthetic effect on goats, and exerted the central anesthesia mechanism by affecting the NO/cGMP signal transduction system and the expression of various neurotransmitters in the brain and hippocampus.