番木瓜优质组培苗生产体系的建立

用含有100mg/LVc＋1mg/LAgNO;+20mg/LPVP液体处理成龄番木瓜（CricappayaL）侧芽，再用70%酒精浸泡50s、0.15%升汞消毒5min，经消毒的外植体接种于MS+KT0.5mg/L+NAA0.2mg/L+GA31.0mg/L＋30g/L蔗糖+7g/L琼脂（pH=5.7），26~28℃，每日光照培养12h，光照强度为15001x，连续培养20d;外植体经初始培养后，继代接种于MS＋BA0.5mg/L+NAA0.1 mg/L+GA，1.0mg/L+蔗糖30gL＋琼脂7g/L（pH=5.7），26~28℃，每日光照培养16h，光照强度为2000Ix，连续培养40d，繁殖系数达3~5倍;继代芽接种MS+BA0.2mg/L+KT0.3mg/L+NAA0.Img/L+NAA0.1 mg/L+GA;1.0 mg/L+ADS40mg/L＋蔗糖30g/L＋琼脂7g/L（pH=5.7），26~28℃，每日光照培养16h，光照强度为20001x，连续培养20d进行壮苗培养，经壮苗培养芽接种于MS+KT0.1~0.2mg/L+ NAA0.05~0.1mg/L+ IBA0.2-0.3mg/L+蔗糖20~30gL+琼脂6.5g/L（pH=5.6），26~28℃，每日光照培养12h，光照强度为1500kx，连续培养 15~20d 进行催根培养，生根率达85%以上。生根苗经2~3d的自然光炼苗后，移栽于沙土∶椰糠∶菜园土质量比为1∶1∶1 混和的基质中，移苗后1周内，每天喷施浓度为200～400mg/L的IBA，移栽成活率达80%以上。笔者就目前番木瓜组培快繁中的问题作了系统的研究，建立了一套适合番木瓜优质种苗生产的技术体系。

Establishment of a Tissue Culture System for Papaya

Papaya is a giant herbaceous plant and has an important value for food industry. It has been grown in tropical and subtropical regions of the world for a long time.An attempt was made to establish a tisue culture sys-tem for rapid propagation of papaya.Mature buds were treated with a solution of 100mg/L.PVP+1 mg/LVc+20mg/L. AgNO,then dipped in 70%(V/)of ethanol for 15S, treated with 0.15%(V/V) of mercury(Hg)chloride for5min. These buds were then culured on MS medium(pH=5.7) supplemented with KT0.5mg/L,NAA0.2 mg/L,GA41.0, sucrose 30g/L and agar7g/L under the condition of 26~28Cand 1 500k,20h per day for 20d.The explants, when initally cultured, were subeultured on the MS medium(pH=5.7) including BA0.5 mg/L,NAA0.I mg/L, GA,1.0 mg/L,sucrose30 g/L.and agar7g/Lunder the condition of 26~28Cand2000k,16h perday for40 d. Shoots were grown on the MS medium containing BA0.2 mg/L,KT0.3mg/L,NAA0.I mg/L,NAA0.1 mg/L, GA31.0mg/L.ADS40 mg/L,sucrose30 g/L+agar7 g/L(pH=5.7)uder the condition of26~-28Cand 2000k, 16 h per day for20d. The rols of propagated buds were induced on the MS medium(pH=5.7)induding KT 0.1-0.2 mg/L,NAA0.05~0.I mg/L,IBA0.2-0.3 m/L,sucrose20~30 g/L and agar7 g/L underthe condition of 26~28°C and 1500k,12 h per day for 15~20 d.The inducedroing rate was up to 85%.Then roted planlets were hardened of fr2-3days, transfered into pots containing mixture of humusich soil, coir dust and sandy soil(w:w:w=1:1:1) and then over 80% of the plantlets survived with 200~400 mg/L of IBA being spraied with being sprained 200~400mg/L of IBA.A tisue culure system for rapid propagation of papaya young plants was established.