黑曲霉产木聚糖酶的固态发酵条件优化

目的]研究培养基组成和培养条件对黑曲霉固态发酵产木聚糖酶的影响。方法]以木聚糖酶酶活为评价指标,利用单因素试验和L9（34）正交试验考察摇瓶发酵条件下培养基组成、pH值、培养时间、培养温度和接种量对黑曲霉产生木聚糖酶的影响。结果]最佳培养基组成为：麸皮与玉米芯质量比5∶3,（NH4）2SO4、KH2PO4、CaCl2和MgSO4相对于固体料的质量百分数分别为1.0%、0.2%、0.1%和0.1%）,料液比1∶1.7;最优培养条件为：pH值7.5、培养温度28℃、培养时间60h,其间翻曲2～3次;在此工艺条件下,木聚糖酶的活力可达14698.21IU/g。结论]该优化条件为木聚糖酶的工业化生产和应用提供了依据。

Optimization of Culture Conditions for Xylanase Production by the Solid-state Fementation with Aspergillus niger

Objective]To investigate the solid fermentation for xylanase production by Aspergillus niger.Method] The single-factor tests and orthogonal experiment were employed to evaluate the optimal fermentation conditions for xylanase production by solid-state fermentation with Aspergillus niger.Result]The optimum conditions for producing xylanase are as follows:the ratio of wheat bran:cob in medium 5∶3,(NH4)2SO4 1.0 %,KH2PO4 0.2%,CaCl2 0.1%,MgSO4 0.1%,the ratio of solid to fluid 1∶1.7,the inoculum 1.0%,the initial pH 7.5,the temperature 28 ℃,and the time for fermentation 60 h.Under those conditions,the enzyme activity of xylanase roduced by the strain reached 14 698.21 IU/g dry medium.Conclusion]This study was meaningful for the production and application of xylanase in industry.