| 根蘖型苜蓿根部RNA提取方法的比较 |
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| 引用本文： | 李岩,王铁梅,卢欣石. 根蘖型苜蓿根部RNA提取方法的比较[J]. 草业科学, 2010, 27(5): 67-71. DOI: 10.3969/j.issn.1001-0629.2010.05.012 |
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| 作者姓名： | 李岩 王铁梅 卢欣石 |
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| 作者单位： | 北京林业大学草地资源与生态实验室,北京,100083;北京林业大学草地资源与生态实验室,北京,100083;北京林业大学草地资源与生态实验室,北京,100083 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家科技支撑项目子课题 |
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| 摘 要： | 以根蘖型苜蓿Medicago sativa的根、茎、叶为材料,利用紫外分光光度计和凝胶电泳法比较了改良CTAB法和TRNzol试剂盒法等4种方法提取的苜蓿总RNA的产率和纯度。结果表明:改良CTAB法提取的RNA的OD260nm/OD230nm大于2.0,并且OD260nm/OD280nm接近1.8,具有较高的纯度。凝胶电泳结果表明,改良CTAB法有28S rRNA和18S rRNA 2条清晰的条带,且很少有降解;TRNzol试剂盒法获得的RNA品质也较好,但存在降解和弥散现象;其他2种方法提取效果较差。这证明改良CTAB法提取的总RNA具有很高的纯度,可以满足进一步分子生物学研究的要求;TRNzol试剂盒法也可以满足实验的下一步要求,但需要进一步纯化。
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| 关 键 词： | 根蘖型苜蓿 总RNA 提取方法 |
Comparative study on extraction methods of total RNA from creeeping-rooted alfalfa |
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| LI Yan,WANG Tie-mei,LU Xin-shi. Comparative study on extraction methods of total RNA from creeeping-rooted alfalfa[J]. Pratacultural Science, 2010, 27(5): 67-71. DOI: 10.3969/j.issn.1001-0629.2010.05.012 |
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| Authors: | LI Yan WANG Tie-mei LU Xin-shi |
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| Affiliation: | LI Yan,WANG Tie-mei,LU Xin-shi(Grassland Resources and Ecology Laboretry,Beijing Forestry University,Beijing 100083,China) |
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| Abstract: | Total RNA from root,stem and leaf of Creeeping-rooted alfalfa were extracted with four methods,including a modified CTAB method,TRNzol method and other two methods,and its quality and quantity were compared.The results indicated that the modified CTAB method was better than the original method since the value of OD260 nm/OD230 nm and OD260 nm/OD280 nm were 2.0 and 1.8,the purities were great higher,the bands of 28S and 18S rRNA on Gel eletro pH oresis were very clear and did not degradated.Comparably,TRNzol... |
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| Keywords: | creeeping-rooted alfalfa total RNA extract methods |
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