| 玉米C型胞质不育恢复基因Rf4的RFLP定位 |
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| 引用本文： | 汤继华,季洪强,黄中文,季良越,王承花. 玉米C型胞质不育恢复基因Rf4的RFLP定位[J]. 河南农业大学学报, 2001, 35(2): 99-102 |
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| 作者姓名： | 汤继华 季洪强 黄中文 季良越 王承花 |
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| 作者单位： | 河南农业大学,;河南职业技术师范学院,;驻马店地区农校, |
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| 基金项目： | 国家自然科学基金!资助项目 ( 3 9470 45 1) |
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| 摘 要： | 采用田间育性鉴定和RFLP分子标记的方法对玉米C型胞质不育的恢复基因进行了研究 .田间育性结果表明 ,玉米C型胞质不育有两对重叠恢复基因 ,通过对A192近等基因系和 (CMS C2 37×A6 19)BC1分离群体的RFLP分子标记 ,将玉米的C型胞质不育恢复基因Rf4定位在第 8染色体短臂上 ,与RFLP探针npi2 2 0和csu2 9连锁
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| 关 键 词： | 玉米 C型胞质不育 恢复基因 RFLP标记 |
| 文章编号： | 1000-2340(2001)02-0099-04 |
Mapping of the main restoring gene (Rf4)of C-cytoplasmic male sterility in maize with RFLP |
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| TANG Ji hua ,JI Hong qiang ,HUANG Zhong wen ,et al. Mapping of the main restoring gene (Rf4)of C-cytoplasmic male sterility in maize with RFLP[J]. Journal of Henan Agricultural University, 2001, 35(2): 99-102 |
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| Authors: | TANG Ji hua JI Hong qiang HUANG Zhong wen et al |
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| Affiliation: | TANG Ji hua 1,JI Hong qiang 1,HUANG Zhong wen 2,et al |
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| Abstract: | A study was conducted on the restoring gene(Rf4 ） of C-cytoplasmic male sterility in maize by means of field fertility determinat ion and RFLP molecular marker.Feild fertility results show that there were two p airs of overlapping restoring genes of C-cytoplasmic male sterility in maize.Us ing the method of near-isogenic line A192 and the BC1 segregating populatio n of (CMS-C237×A619） restriction fragment length polymorphism(RFLP),the re stor ing gene Rf4 for C-cytoplasmic male sterility was localized in chromosome 8 S,linking to two RFLP markers loci.npi220 and csu29. |
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| Keywords: | maize the male sterility of C cytoplasmic restoring genes RFLP marker |
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