新疆褐牛瘦素基因在不同部位中表达水平与肉质指标的关联性

【目的】肉品质与物种的遗传因素具有一定的关联性。新疆褐牛瘦素基因在不同部位中表达水平与肉质指标的关联性,为牛肉属性差异的品质标识提供参考。【方法】选取新疆褐牛为研究对象,采用荧光定量聚合酶链式反应(qRT-PCR)技术测定新疆褐牛皮下、肌间、肾周、心周4个部位脂肪组织中的瘦素基因(leptin)的表达水平,检测活重、胴体指标、剪切力、蒸煮损失等肉质性状,分析新疆褐牛在这4个部位脂肪组织中leptin基因表达水平与所测肉品质指标的关联性。【结果】新疆褐牛不同部位leptin基因相对表达量由低到高依次为心周脂肪、肾周脂肪、皮下脂肪和肌间脂肪。皮下脂肪组织中leptin表达水平与其背最长肌组织中的剪切力、蒸煮损失、肌内脂肪含量显著正相关,相关系数分别为0.555、0.479、0.474(P<0.05)。【结论】瘦素蛋白在新疆褐牛脂肪组织中内普遍存在,leptin基因可能参与新疆褐牛肉质形成过程,可作为研究新疆褐牛肉质的一个候选基因。

Correlation between Leptin Gene Expression Level in Different Tissues and Meat Quality in Xinjiang Brown Cattle

【Objective】 To better understand the close association between meat quality and genetic factors of animal species.【Method】 Xinjiang brown cattle were chosen as the research object, and the expression levels of leptin gene (leptin) in subcutaneous fat, intermuscular fat, perirenal fat, and pericardium fat were determined by quantitative real-time fluorescence polymerase chain reaction (qRT-PCR).The live weight, carcass index, shear force, cooking loss and other meat quality traits were also measured and the correlation between leptin gene expression level and meat quality index in above the three tissues of Xinjiang brown cattle was analyzed.【Results】 The results showed that the relative expression levels of leptin genes in different parts of Xinjiang brown cattle were pericardial fat, subcutaneous fat, and intermuscular fat in descending order.The expression level of leptin in subcutaneous adipose tissue was significantly positively correlated with its outer spine shear force, cooking loss, and intramuscular fat content.The correlation coefficients were 0.555, 0.479, 0.474, respectively (P<0.05).【Conclusion】 It is confirmed that leptin protein is widespread in the adipose tissue of Xinjiang brown beef.Its gene may be involved in the formation process of Xinjiang brown beef, which can be used as a candidate gene for studying Xinjiang brown beef quality thus providing a reference for the quality identification of beef attributes.