| 云引稻瘟病抗性基因型的初步分析及其近等基因系构建 |
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| 引用本文： | 朱永生,蔡秋华,薛伟民,谢鸿光,吴方喜,张建福,谢华安.云引稻瘟病抗性基因型的初步分析及其近等基因系构建[J].福建农业学报,2012(1):1-6. |
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| 作者姓名： | 朱永生 蔡秋华 薛伟民 谢鸿光 吴方喜 张建福 谢华安 |
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| 作者单位： | 福建省农业科学院水稻研究所;农业部华南杂交水稻种质创新与分子育种重点实验室/福州国家水稻改良分中心/福建省作物分子育种工程实验室/福建省水稻分子育种重点实验室;福建省作物种质创新与分子育种省部共建国家重点实验室培育基地;水稻国家工程实验室 |
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| 基金项目： | 国家“863”计划项目(2011AA10A101、2010AA101801、2010AA101804、2006AA100101);国家自然科学基金项目(30871509);福建省自然科学基金重点项目(2007J0005);福建省杰出青年基金项目(2009J06011);福建省自然科学基金项目(2010J01102、2010J01103);福建省财政专项——福建省农业科学院科技创新团队项目(STIF-Y04) |
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| 摘 要： | 利用水稻品种稻瘟病抗性基因型鉴定菌株RB01～RB22,对云引苗期叶片进行离体接种,结果表明云引除对RB17和RB20感病外,对其他菌株均表现抗病,通过分析RB01～RB22对目前生产上广泛应用的24个稻瘟病抗性基因的抗性鉴定结果表明,这些基因的反应型均与云引不同,即可能含有除上述抗病基因之外的新的稻瘟病抗原。此外,通过连续回交、分子标记辅助选择和人工注射接菌相结合的方法,构建了以普感稻瘟病品种丽江新团黑谷(LTH)为遗传背景,含有抗稻瘟病菌株"四川-43"基因的近等基因系,为该抗病基因的遗传学研究和应用构建了良好的载体。
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| 关 键 词： | 稻瘟病 抗病基因 鉴定 近等基因系 |
Preliminary Genotype Analysis of Broad-spectrum Rice Blast Resistance Variety Yunyin and its Near-isogenic Lines Construction |
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| ZHU Yong-sheng,CAI Qiu-hua,XUE Wei-min,XIE Hong-guang,WU Fang-xi,ZHANG Jian-fu,XIE Hua-an.Preliminary Genotype Analysis of Broad-spectrum Rice Blast Resistance Variety Yunyin and its Near-isogenic Lines Construction[J].Fujian Journal of Agricultural Sciences,2012(1):1-6. |
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| Authors: | ZHU Yong-sheng CAI Qiu-hua XUE Wei-min XIE Hong-guang WU Fang-xi ZHANG Jian-fu XIE Hua-an |
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| Institution: | 1,2,3,4(1.Rice Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350019,China;2.Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China,Ministry of Agriculture,P.R China/Fuzhou branch,National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/ Fujian Key Laboratory of Rice Molecular Breeding,Fuzhou,Fujian 350003,China;3.Incubator of National key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology,P.R.China,Fuzhou,Fujian 350003, China;4.National Rice Engineering Laboratory of China,Fuzhou,Fujian 350003,China) |
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| Abstract: | The phenotype of broad-spectrum rice blast resistance variety Yunyin was analyzed and identified by inoculated with rice blast fungi RB01-RB22 of known avirulence genes as pathogen in vitro.The results showed that Yunyin was resistant to all rice blast fungi except RB17 and RB20.Analyzing the widely used 24 resistance genes in production at present,the results showed that Yunyin may contain new blast resistance genes besides these genes,and there were able to be applied for rice breeding and production.In addition,in this study,we constructed near-isogenic lines with LTH as genetic background and contained blast-resistant gene to blast isolate SiChuan 43 by successive backcrossing combining marker-assisted selection with artificial inoculation.These works would be beneficial to the genetics study and application of this blast-resistance gene. |
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| Keywords: | rice blast resistance gene identification near-isogenic lines |
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