| 欧文氏杆菌CXJZ11-01基因组文库的构建 |
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| 引用本文： | 石君,刘正初,成莉凤,段盛文,郑科,冯湘沅,胡镇修.欧文氏杆菌CXJZ11-01基因组文库的构建[J].湖北农业科学,2008,47(6). |
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| 作者姓名： | 石君 刘正初 成莉凤 段盛文 郑科 冯湘沅 胡镇修 |
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| 作者单位： | 中国农业科学院麻类研究所,长沙,410205 |
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| 基金项目： | 国家"863"计划项目 |
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| 摘 要： | 采用改良鸟枪法构建了草本纤维精制高效菌种CXJZ11-01的基因组文库,通过透明圈法筛选到了2个木聚糖酶基因阳性克隆.
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| 关 键 词： | 欧文氏杆菌CXJZ11-01 基因组文库 木聚糖酶基因 |
Genomic Library Construction of Erwinia carotovora CXJZ11-01 |
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| SHI Jun,LIU Zheng-chu,CHENG Li-feng,DUAN Sheng-wen,ZHENG Ke,FENG Xiang-yuan,HU Zhen-xiu.Genomic Library Construction of Erwinia carotovora CXJZ11-01[J].Hubei Agricultural Sciences,2008,47(6). |
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| Authors: | SHI Jun LIU Zheng-chu CHENG Li-feng DUAN Sheng-wen ZHENG Ke FENG Xiang-yuan HU Zhen-xiu |
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| Institution: | SHI Jun,LIU Zheng-chu,CHENG Li-feng,DUAN Sheng-wen,ZHENG Ke,FENG Xiang-yuan,HU Zhen-xiu (Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha 410205,China) |
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| Abstract: | By using shot-gun method,the genomic DNA library of the efficient herbaceous extraction strain E.carotovora CXJZ11-01 was constructed.Using the method of transparent circle to the positive clones, the identical xylanase genes was identified. |
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| Keywords: | E carotovora CXJZ11-01 genomic library xylanase gene |
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