| 槟榔基因组DNA的高效提取方法 |
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| 引用本文： | 曾莉娟,李涛,张雨良,刘志昕.槟榔基因组DNA的高效提取方法[J].江西农业学报,2010,22(2):58-59,63. |
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| 作者姓名： | 曾莉娟 李涛 张雨良 刘志昕 |
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| 作者单位： | 1. 中国热带农业科学院,热带生物技术研究所,海南,海口,571710;中国热带农业科学院,科技信息研究所,海南,儋州,573717
2. 中国热带农业科学院,热带生物技术研究所,海南,海口,571710 |
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| 基金项目： | 国家科技支撑计划课题“热带农林重要有害生物检测监测预警技术研究”(2007BAD48B01) |
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| 摘 要： | 以槟榔叶片为植物材料,采用改良的SDS-CTAB方法提取其基因组DNA。结果表明,以水饱和酚代替Tris饱和酚对样品进行抽提的SDS-CTAB法可以获得纯度高(OD260/OD280在1.7~1.9、OD260/OD230大于2)、一级结构完整、分子量大于23 kb的DNA,产率在50~120μg/g。该法所提DNA成功用于后续的分子生物学研究,并在花生、香蕉等植物上得到应用。
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| 关 键 词： | 槟榔 基因组DNA SDS-CTAB |
High-efficient Extraction of Genomic DNA from Areca catechu L. |
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| ZENG Li-juan,LI Tao,ZHANG Yu-liang,LIU Zhi-xin.High-efficient Extraction of Genomic DNA from Areca catechu L.[J].Acta Agriculturae Jiangxi,2010,22(2):58-59,63. |
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| Authors: | ZENG Li-juan LI Tao ZHANG Yu-liang LIU Zhi-xin |
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| Institution: | 1.Institute of Tropical Bioscience and Biotechnology/a>;Chinese Academy of Tropical Agricultural Sciences/a>;Haikou 571710/a>;China/a>;2.Institute of Scientific and Technological Information/a>;Danzhou 573717/a>;China |
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| Abstract: | The extraction of genomic DNA from the leaves of Areca catechu Linnaeus was researched by optimized SDS-CTAB method in order to obtain high-quality genomic DNA for subsequent molecular biological experiment.The results showed that high-quality DNA was isolated,the OD260/OD280 of the samples was between 1.7 and 1.9,the OD260/OD230 of the samples was more than 2,its primary structure was perfect,its molecular weight was more than 23 kb,and its yield was in range of 50～120 μg/g.The DNA extracted by this method was successfully applied in the subsequent molecular biological experiment.The method was also applied to the extraction of DNA from peanut,banana and so on. |
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| Keywords: | Areca catechu Gnomic DNA SDS-CTAB |
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