云南松口蘑的 ＩＳＳＲ遗传多样性研究

本文利用 １６条 ＩＳＳＲ引物，对来自云南香格里拉、大理、楚雄和昆明的 ４个松口蘑居群共 ４２个个体的遗传多样性进行研究。结果表明，ＩＳＳＲ ＰＣＲ共扩增出 ７６条条带；总多态性位点百分率 ＰＰＢ＝６１０４％，多态位点百分率依次为 ５６７６％，６７７５％，７０２７％和 ５０００％；总的基因多样度指数 （Ｈｔ）为０３２１５，居群内的 Ｎｅｉ遗传多样度指数 （Ｈｓ）为０２４４７，Ｓｈａｎｎｏｎ多样性指数 （Ｉ）为 ０４７１７，居群间的遗传分化系数Ｇｓｔ为０２４７１，基因流 Ｎｍ为１５２３４，这说明云南松口蘑具有较高的遗传多样性，且主要存在于居群之间，居群内的变异较小；遗传距离表明 ４个居群中香格里拉和昆明的亲缘关系最远，大理和楚雄的亲缘关系最近。

The Genetic Diversity of Tricholoma matsutake by ISSR in Yunnan Province

Genetic diversity of 42 individuals belonging to four populations of Tricholoma matsutake from Shangri la, Dali, Chuxiong and Kunming in Yunnan Province was detected by using 16 ISSR primers. The results showed that 76 DNA bands were amplified by 16 primers above mentioned and the total percentage of polymorphic bands (PPB) was 61.04%.The PPBs of Shangri la, Dali, Chuxiong and Kunming were 56.76%, 67.75%, 70.27% and 50-00%, respectively.The total genetic diversity (Ht) was 0.3215, the genetic diversity within population (Hs)was 0.2447, Shannon′s diversity index (I) was0.4717, the coefficient of gene variation was 0.2471 and the gene flow among populations was 1.5234 It was implied that a higher genetic diversity of T matsutake mainly existed among populations, and a lower degree of genetic variation occurred within populations. The genetic relationship was the farthest between Shangri la and Kunming, and it was the closest between Dali and Chuxiong in the genetic distance.