珍珠龙胆石斑鱼血细胞发生的研究

用瑞氏染液对珍珠龙胆石斑鱼的头肾、体肾、脾脏等器官组织的涂片、印片进行染色、观察、测定和统计,以研究血细胞发生。试验结果显示,红细胞的发育经历4个阶段,分别是原红细胞、幼红细胞、红细胞和衰老红细胞,细胞体积逐渐变小;粒细胞的发育经历5个阶段,分别是原粒细胞、早幼粒细胞、中幼粒细胞、晚幼粒细胞和成熟粒细胞,中性粒细胞的体积由大变小再变大,嗜酸性粒细胞体积逐渐变小;单核细胞和淋巴细胞的发育经历3个阶段,分别是原始、幼稚和成熟,细胞体积逐渐变小。珍珠龙胆石斑鱼的各种细胞体积均较其他鱼类的细胞体积大;中性粒细胞的体积与嗜酸性粒细胞的体积差异较大。脾脏中未观察到原红细胞和原粒细胞,仅在体肾中观察到巨噬细胞和网状细胞。头肾是珍珠龙胆石斑鱼最主要的造血器官,体肾次之。头肾和体肾是珍珠龙胆石斑鱼红细胞、粒细胞的主要发生器官;头肾、体肾、脾脏是原单核细胞、原淋巴细胞发生的主要场所。

Hematogenesis in Grouper Hybrid Epinephelus lanceolatus♂×E.fuscoguttatus♀

The smear and print of head kidney, body kidney, and spleen of grouper hybrid Epinephelus lanceolatus ♂ × E. fuscoguttatus ♀ were stained with Wright′s dye solution, and the blood cells were observed, photographed, measured, and statistically processed to probe into the development of haemocytes. The results showed that the development of red blood cells was divided into four stages: namely proerythroblast, immature erythroblast, erythrocyte and old erythrocyte, in which the cell volume was getting smaller during the maturation of the erythroid cells. The granuloid lineages were divided into five stages: progranulocytes, promyelocytes, mesenchymal granulocytes, late granulocytes and mature granulocytes, in which the cell volume was getting smaller, however, from the neutrophilic metagranulocyte to the neutrophilic granulocyte, the their sizes were larger than the cells of other fish from the progranuloblast to the neutrophilic metagranulocyte. During the maturation of the eosinophilic granulocytes, they were reduced in size. The monocytoid lineage and lymphoid lineage were divided into three stages: primitive stages, immature stage and mature stage, and these two kinds of cells had regularly reduced in size during development. There were larger cells in the grouper hybrid than those in other fishes, much larger neutrophilic granulocyte than eosinophilic granulocyte, without proerythroblast and progranuloblast in spleen. Only macrophage and reticulocyte were oberved in body kidney. The developments of erythrocyte, granulocyte, lymphocyte, and monocyte were found mainly in head kidney, and then in body kidney. Erythrocytes, and granulocytes developed mainly in head kidney and body kidney. The findings indicated that head kidney, body kidney, and spleen were the main sites of genesises of primary monocytes and protolymphocytes.