南极适冷菌Rheinheimera sp.97产抗菌活性物质发酵条件的优化

目的]优化南极适冷菌Rheinheimera sp.97产抗菌活性物质的发酵条件。方法]通过单因素和正交试验对南极适冷菌R.sp.97产抗菌活性物质的发酵培养基进行了优化,并通过单因素试验对其发酵条件进行了优化。结果]菌株R.sp.97产抗菌活性物质的最佳培养基为：蛋白胨3.0g/L,硫酸铵1.0g/L,淀粉2.0g/L,NaCl15.0g/L;最佳发酵条件为：发酵培养基初始pH8,发酵温度10℃,摇瓶装液量30.0%（V/V）,接种量1.0%（V/V）。优化后菌株R.sp.97发酵液的抗菌活性较优化前提高了18.1%。结论]为研究南极适冷菌R.sp.97的活性物质奠定了基础。

Optimization of Fermentation Medium and Conditions of Antibiotic Active Substances Produced by Antarctic Psychrotrophic Bacterium Rheinheimera sp. 97

Objective] The aim was to optimize the fermentation medium and conditions of antibiotic active substances produced by Antarctic psychrotrophic bacterium Rheinheimera sp.97.Method] Single-factor experiment and orthogonal test were adopted to optimize the fermentation medium of antibiotic active substances produced by Antarctic psychrotrophic bacterium R.sp.97,while the fermentation conditions were optimized by single-factor experiment.Result] The optimum fermentation medium for the antibiotic active substances production was as follows:tryptone 3.0 g/L,ammonium sulfate 1.0 g/L,starch 2.0 g/L,NaCl 15.0 g/L.The optimized fermentation conditions were as follows:the starting pH of medium was 8.0,fermentation temperature was 10 ℃,liquid volume in Erlenmeyer flask was 30 %(V/V)and inoculation amount was 1%(V/V).Under the optimized fermentation medium and conditions,the antibacterial activity of R.sp.97 was increased by 18.1%.Conclusion] This study had provided basis for the antibiotic active substances produced by Antarctic psychrotrophic bacterium R.sp.97.