低能氮离子注入对同源四倍体水稻双胚苗特性的影响

利用低能离子注入技术对同源四倍体水稻进行注入处理,在其后代群体内筛选到具有双胚苗特征的突变单株。在突变单株的纯化过程中对其多胚苗性状的表现进行了观察鉴定。随后,在突变体后代的主要农艺性状趋于稳定时对两个株系在不同温度条件下的多胚苗频率进行了鉴定。结果表明,利用离子注入技术可以对同源四倍体水稻进行有效地改良,由此可以获得具有遗传变异特点的新种质,其双胚苗特性随着自交世代的推进而得到保持。同源四倍体双胚苗材料在其性状表达特征、性状表达频率和性状表达条件等方面均显现出一定的特异性。在试验材料的群体内多胚苗发生频率因材料种类不同或发芽温度不同而表现出一定的差异性。种子经过去颖壳处理后可以使其双苗频率提高32.4%~36.0%。在同源四倍体双胚苗材料中,其苗位特征表现出明显的多样性。

Characteristic Stability of Autotetraploid Twin-embryo Seedling Rice after Low-energy N+ Ion Beam Implantation

The off-springs of autotetraploid rice were treated by lowenergy N ion beam,then the mutant seedlings with twin-embryo character was elected.When the main agronomic characters of the twin-embryo seedling plants trends to be stable,two lines were selected to study the frequencies of twin-embryo seedlings in different temperatures.The results showed that autotetraploid rice might be effectively improved by ion beam bio-technique,from which the new germplasm with hereditable twin-embryo seedling could be selected.The twin-embryo seedling plants showed obviously the specificity in the expressing feature,frequency and condition of goal traits.The frequencies of twin-embryo seedlings were viarious in different experimental material and different germinating temperatures.The frequency of twin-embryo seedlings could be increased by 32.4%-36.0% by husking the seed hull.The morphological characteristics of twin-embryo seedling plants presented apparent multiplicity.