| 低温胁迫对甘薯S-腺苷甲硫氨酸合成酶mRNA表达水平的影响（英文） |
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| 作者单位： | YUE Chang-wu1，XIAO Jing2，LING Xin1，ZENG Ni1 1.Central Laboratory of Zunyi Medical College，Zunyi 563000;2.Morphologic Laboratory of Zunyi Medical College，Zunyi 563000 |
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| 摘 要： | Objective] The mRNA expression level changes of S-adenosylmethionine synthetase(SAMS)under low temperature stress was studied.Method]Total RNA were extracted from leaves,stem and earthnut of sweet potato 0,12,24,48 and 72 h after low temperature treatement.mRNA expression level was analyzed by reverse expression and Real-time PCR technique.Result]The expression quality of the gene extracted from leaves,stem and earthnut increased and the expression quality reached the peak point 24,72 and 72 h after low temperature treatment respectively.The expression change of earthnut was the biggest.Conclusion]Low temperature was good for increasing mRNA expression of relevart genes.
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Effect of Low Temperature Stress on Sweet Potato S-Adenosyl Methionine Synthetase Gene Expression |
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| Authors: | YUE Chang-wu XIAO Jing LING Xin ZENG Ni Central Laboratory of Zunyi Medical College Zunyi Morphologic Laboratory of Zunyi Medical College Zunyi |
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| Institution: | YUE Chang-wu1,XIAO Jing2,LING Xin1,ZENG Ni1 1.Central Laboratory of Zunyi Medical College,Zunyi 563000,2.Morphologic Laboratory of Zunyi Medical College,Zunyi 563000 |
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| Abstract: | Objective] The mRNA expression level changes of S-adenosylmethionine synthetase(SAMS)under low temperature stress was studied.Method]Total RNA were extracted from leaves,stem and earthnut of sweet potato 0,12,24,48 and 72 h after low temperature treatement.mRNA expression level was analyzed by reverse expression and Real-time PCR technique.Result]The expression quality of the gene extracted from leaves,stem and earthnut increased and the expression quality reached the peak point 24,72 and 72 h after low temperature treatment respectively.The expression change of earthnut was the biggest.Conclusion]Low temperature was good for increasing mRNA expression of relevart genes. |
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| Keywords: | Low temperature stress Ipomoea batats L S-adenosyl methionine synthetase Real-time PCR |
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