| 高速逆流色谱分离纯化连翘中的两对立体异构体 |
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| 引用本文： | 段文娟,耿岩玲,庄会永,傅茂润,刘建华,王晓.高速逆流色谱分离纯化连翘中的两对立体异构体[J].林产化学与工业,2011,31(5):41-47. |
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| 作者姓名： | 段文娟 耿岩玲 庄会永 傅茂润 刘建华 王晓 |
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| 作者单位： | 山东省分析测试中心;山东省大型精密仪器应用技术重点实验室,山东济南250014 |
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| 基金项目： | 山东省科技攻关项目(2010GSF10287); 济南市高校院所自主创新计划(201004010); 国家高科技研究发展计划(2010AA101605-05) |
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| 摘 要： | 应用高速逆流色谱法(HSCCC)分离纯化连翘果实中的两对木脂素类立体异构体.分别用乙酸乙酯-正丁醇-甲醇-水(体积比5∶0.7∶1∶5)和乙酸乙酯-正丁醇-甲醇-水(体积比5∶0.5∶1∶5)为两相溶剂体系,在转速为800r/min、流速为1.5 mL/min、检测波长为254 nm的条件下进行分离,所得馏分经高效液相...
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| 关 键 词： | 高速逆流色谱 连翘 立体异构体 |
Preparative Isolation and Purification of Two Pairs ofStereomer from the Fruits of Forsythia suspensa by High-speed Counter-current Chromatography |
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| DUAN Wen-juan,GENG Yan-ling,ZHUANG Hui-yong,FU Mao-run,LIU Jian-hua,WANG Xiao.Preparative Isolation and Purification of Two Pairs ofStereomer from the Fruits of Forsythia suspensa by High-speed Counter-current Chromatography[J].Chemistry & Industry of Forest Products,2011,31(5):41-47. |
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| Authors: | DUAN Wen-juan GENG Yan-ling ZHUANG Hui-yong FU Mao-run LIU Jian-hua WANG Xiao |
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| Institution: | DUAN Wen-juan,GENG Yan-ling,ZHUANG Hui-yong,FU Mao-run,LIU Jian-hua,WANG Xiao(Shandong Analysis and Test Center,Key laboratory for Applied Technology of SophisticatedAnalytical Instrument of Shandong Province,Jinan 250014,China) |
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| Abstract: | Two pairs of stereomeric lignan,namely (+) pinoresinol monomethyl ether-,β-D-glucoside and phillyrin,(+) pinoresinol-β-D-glucoside and (+) epipinoresinol-β-D-glucoside,were successfully isolated and purified from the fruits of Forsythia suspensa by high-speed counter-current chromatography (HSCCC) using two-phase solvent systems with composed of ethyl acetate-n-butanol-methanol-water (5 ∶ 0.7 ∶ 1 ∶ 5,volume ratio) and ethyl acetate-n-butanolmethanol-water (5 ∶ 0.5 ∶ 1 ∶ 5,volume ratio ),respectively.The purities of the isolated compounds were all above 97 ％ determined by high-performance liquid chromatography (HPLC) and their structures were confirmed by electrospray ionization MS (ESI-MS),1HNMR,and 13CNMR. |
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| Keywords: | high-speed counter-current chromatography Forsythia suspensa stereomer |
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