种植密度和施氮水平与白肋烟叶片组织结构的关系

用切片显微观察的方法,探索种植密度与施氮水平下,白肋烟各部位叶片组织结构的变化。结果表明,叶片厚、栅栏组织厚、海绵组织厚、组织比、栅栏组织厚/叶片厚、上表皮厚、栅栏组织细胞宽与种植密度呈显著或极显著负相关,三部位平均叶片厚、上下表皮厚、栅栏组织细胞宽降率从15000￣18000株/hm2>18000￣21000株/hm2,栅栏组织厚、海绵组织厚、组织比、栅栏组织厚/叶片厚降率15000￣18000株/hm2<18000￣21000株/hm2;与施氮量呈显著或极显著正相关,三部位平均叶片厚、海绵组织厚、下表皮厚增率240￣300kg/hm2>300￣360kg/hm2,栅栏组织厚、组织比、上表皮厚、栅栏组织厚/叶片厚、栅栏组织细胞宽增率则是240￣300kg/hm2<300￣360kg/hm2,密度因素对三部位叶片组织结构(平均)影响效应,叶片厚、栅栏组织厚、组织比、上下表皮、栅栏组织细胞宽大于施氮因素,而海绵组织厚小于施氮因素,两因素对栅厚/叶厚影响效应均等。综合试验结果,种植密度18000￣19500株/hm2,氮用量270￣300kg/hm2较为适宜。

Relationship of Planting Density, Rate of N Application and Tissue Structure of Burley tobacco Leaf

With different planting density and rate of N application,the tissue structure of burley tobacco leaf was studied by the micro-observation of slice.The results showed that the thickness of leaf,palisade tissue,spongy tissue and upper epidermis,ratios of tissue,thickness of palisade tissue /leaf thickness,and the width of palisade tissue had significantly negative correlation with planting density.The rate of descending in average thickness of leaf,upper epidermis and under epidermis,and the width of palisade tissue increased in the planting density from 15000~18000/hm2 to 18000~21000/hm2.While the rate of descending in average thickness of palisade tissue and spongy tissue,ratios of tissue,and thickness of palisade tissue /leaf thickness descended.But this had significantly positive correlation with rate of N application.The rate of rising in average thickness of leaf,spongy tissue and under epidermis increased at rate of N application from 240~300kg/hm2 to 300~360kg/hm2.While the rate of rising in average thickness of palisade tissue and upper epidermis,ratios of tissue,thickness of palisade tissue /leaf thickness,and the width of palisade tissue descended The effect on the thickness of leaf,under epidermis spongy tissue and upper epidermis,ratios of tissue,and the width of palisade tissue in planting density was higher than rate of N application,but the thickness of spongy tissue was lower.The thickness of palisade tissue /leaf thickness was same.With this result,the planting density of 18000~19500/hm2 and rate of N applicationfrom 270~300kg/hm2 was feasible in burley tobacco.