甜樱桃品种SSR指纹图谱数据库的建立

为了建立甜樱桃品种指纹图谱数据库,从而对不同甜樱桃品种进行分子鉴定,以"红灯"、"萨米脱"、"吉塞拉5号"、"吉塞拉6号"等24个甜樱桃主要栽培品种为试材,利用2.5%琼脂糖凝胶电泳进行SSR引物的筛选,选择差异明显且等位基因数目少(2个)的SSR标记,从38对SSR引物中筛选出能够扩增出稳定带型且不同材料之间差异明显的SSR引物,并从中选出10对SSR引物进行甜樱桃分子指纹分析,根据各甜樱桃DNA样品的电泳条带结果进行赋值,利用Visual Basic和Ac-cess软件进行数据库编程,创建一个数据库,入选的10对引物对样品的扩增结果赋值排列起来,即成为甜樱桃的"基因身份证"号码,从而根据分子身份证对不同甜樱桃种质进行鉴别。

Construction of Molecular Fingerprinting Database for Sweet Cherry using SSR Markers

SSR primers were developed from the 24 cultivars of sweet cherry such as ''Hongdeng'', ''Summit'', ''Jisaila 5'', ''Jisaila 6'' by 2.5% agar gel electrophoresis. The aim is to select the SSR markers with significant selective difference and a few alleles (2 alleles). The SSR primers, which can amplify stable and distinct band type between different materials, were selected from the 38 pairs of primers, and 10 primer pairs were used to analyze the fingerprint of sweet cherry. The electrophoresis results of the 24 sweet cherry DNA samples were evaluated and analyzed with the softwares of Visual Basic and Access, and the database was established. The amplified bands of the selected 10 primer pairs were lined up, which became the gene identification number of sweet cherry. Eventually, the sweet cherry germplasms were identified according to their molecular identification.