莴苣花叶病毒北京分离物基因组3'末端序列分析 |
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作者姓名: | 尚巧霞 向海英 韩成贵 李大伟 于嘉林 |
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作者单位: | 北京农学院植物科学技术系,中国农业大学植物病理学系农业生物技术国家重点实验室,中国农业大学植物病理学系农业生物技术国家重点实验室,中国农业大学植物病理学系农业生物技术国家重点实验室,中国农业大学植物病理学系农业生物技术国家重点实验室 北京102206,中国农业大学植物病理学系,农业生物技术国家重点实验室,北京100094,北京100094,北京100094,北京100094,北京100094 |
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基金项目: | 国家973计划资助项目(2006CB101903),北京农学院青年科学基金项目(06-ZD-ZK-02) |
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摘 要: | 笔者通过RT-PCR方法对LMV北京分离物(LMV-BJ)基因组3''端1620nt的核苷酸片段进行了克隆和序列分析(GenBank登录号为EF423619)。所获片段含有NIb基因3''端的574nt,编码NIb C-端190个氨基酸;完整的CP基因,全长为834nt,编码一个由277个氨基酸组成的分子量约为30kDa的结构蛋白;3''非编码区含有209nt。通过序列分析软件将LMV-BJ与已经报道的法国分离物O(X97704)、法国分离物E(X97705),美国分离物(X65652),巴西分离物(AJ278854)和余杭分离物(AJ306288)基因组3''末端和CP基因的核苷酸序列与氨基酸序列分别进行了比较。
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关 键 词: | 莴苣花叶病毒 北京分离物 3''端基因组片段 cDNA克隆和序列分析 |
Sequence Analysis of the 3' Terminal Region of the Genome of Lettuce mosaic virus Beijing Isolate |
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Authors: | Shang Qiaoxi Xiang Haiying Han Chenggui Li Dawei Yu Jialin |
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Institution: | 1.Department of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206; 2.Department of Plant Pathology and State Key Laboratory for A grobiotechnology, China Agricultural University, Beijing 100094 |
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Abstract: | The 3'' terminal genomic 1620 nucleotides of the Lettuce mosaic virus Beijing isolate(LMV-BJ) from China was determined(the GenBank accession number is EF423619).The sequence started within a single open reading frame which was expected to encode a part of the C-terminus of the NIb protein of 190 amino acids by 574 nucleotides,complete capsid protein(CP) of 277 amino acids encoded by 834 nucleotides and followed by a 3'' untranslated region(UTR) of 209 nucleotides.The nucleotide and amino acid sequence identity of 3'' terminal regions and cp gene among LMV-BJ and other five previously reported isolates from France O(X97704),France E(X97705),USA(X65652),Brazil(AJ278854),Yuhang(AJ306288)were compared. |
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Keywords: | Lettuce mosaic virus Beijing isolate 3'' terminal genomic region Cloning and sequencing |
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