| 陆两优996种子纯度的SRAP指纹图谱鉴定 |
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| 作者姓名： | 李建军 肖层林 刘志坚 吴升高 |
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| 作者单位： | 湖南农业大学农学院 湖南长沙410128(李建军，肖层林，吴升高)，望城县农业局 湖南望城410200(刘志坚) |
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| 基金项目： | 国家高技术研究发展计划(863计划)，现代农业技术项目(2001AA241023) |
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| 摘 要： | 利用270对SRAP引物对陆两优996和2个亲本的DNA指纹图谱,获得能在父、母本间表现出多态性的特异SRAP标记引物有54对,其中差异性条带136个。利用Me3Em8引物,条带在杂交种完全互补,对200株样本进行了纯度鉴定,结果鉴定纯度为97.50%,与田间种植鉴结果97.50%(海南鉴定)一致,表明SRAP标记技术适用于种子纯度鉴定。
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| 关 键 词： | 两系杂交水稻 陆两优996 SRAP 纯度鉴定 |
Studies on SRAP Fingerprinting in Purity Identification of Hybrid Seeds of Lu Liangyou 996 |
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| Authors: | Li Jianjunt Xiao Cenglin Liu Zhijian Wu Shenggao |
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| Institution: | 1Hunon Agricultural College, H unan Agricultural University, Changsha Hunan 410128; 2Agricultural Bereau of Wangcheng County, Wangcheng Hunan 410200 |
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| Abstract: | In this research, DNA fingerprints of 1 male sterile lines and 1 restorer lines of two-line hybrid rice were constructed by using 270 SRAP (Sequence related amplified polymorphism) primers. The 54 SARP primers could amplify 136 polymorphic bands totally with a mean of 2. 5 bands per primer. The 200 single plants of Luliangyou 996 were tested in laboratory by a specific SRAP marker(Me3Em8)whose bands were complement in hybrid combination seeds. The seed purity was 97.50%, which was same to the field purity (97.50% in Hainan). The results of this research suggested that SRAP technique could be used in seed purity identification of two-line hybrid rice. |
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| Keywords: | Two-line hybrid rice Lu liangyou 996 SRAP (Sequence related amplified polymorphism) Purity identification |
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