药用植物防风组织培养关键技术研究

以防风成熟种子为材料建立无菌系,研究了不同的外植体、不同基本培养基、不同激素配比对愈伤组织诱导的影响、及不同激素配比对愈伤增殖的影响。结果表明:愈伤组织诱导的最佳基本培养基为MS培养基;诱导率最高的为根,达99.3%,但形成的愈伤组织质地较差,叶的诱导率仅次于根,为84.6%,且质地松散,呈淡绿色,适合进行试验研究,在MS 2,4-D0.6 6-BA0.5培养基上,7d即可见愈伤;正交试验结果表明:6-BA、NAA对愈伤组织的增殖有明显的作用;达显著水平。

Experiments on Tissue Culture of Officinal Foliage Sapashnikovia divaricata Gsch Ischk

It was obtained aseptic line derived from seed condition of Sapashnikovia divaricata (Turcz.) Gsch ischk. It studied affected callus shaped by different explant, different basic medium, different proportion of incretion. Different proportion of incretion and bring up number of days effected proliferated callus. Results showed that be sieving the basic medium; the best basic medium was MS substrate when callus by inducement; the root''s inducement percent was highest, it was 99.3%, but it wrought callus quality of a material wasn''t good; the leaf''s inducement percent was only lower than the root''s inducement percent, it was 84.6%, and quality of a material was fall apart, showed pea green, it is fittest to studied. It could appear callus only 7d in MS 2,4-D0.6 6-BA0.5 substrate. Orthogonal plan experimentation Results showed: The 6-BA and NAA had obvious accelerate effect when its callus was proliferated, the proliferated speed was soonest when kept up''s number of days was 30d.