高pH对拟南芥萌发和主根伸长的影响

土壤盐碱化严重影响作物的产量。近年来用模式植物拟南芥研究植物对盐响应分子机理的研究已经取得突破性进展。然而,植物对碱胁迫响应的生理、生化研究相对比较落后,对pH信号及胁迫响应分子机理的研究还比较少。试验以模式植物拟南芥为材料,研究了不同pH值对拟南芥种子萌发及幼苗生长发育的影响。结果表明:pH值过高会抑制种子萌发;随着pH值的逐渐增加,根的相对生长量和幼苗叶绿素含量均呈现先增加后减少的趋势;同时,根尖生长素含量随着pH值升高呈现先增加后减少的趋势,表明生长素含量变化可能在植物对碱胁迫的响应中,尤其是在根伸长过程中起着重要作用。因此,工作将加深人们对植物在碱条件下的生理和生化反应的理解,为揭示碱信号转导途径,筛选培育耐碱品种等提供依据。

High pH Dramatically Affects Seed Germination and Root Growth of Arabidopsis

Increasing areas in saline and alkali soil dramatically affect crop yield.Recent research in salt signal transduction pathway using Arabidopsis have made great process in understanding the genetic basis of plant tolerance to salinity.However,the study on plant response and the molecular mechanisms to alkali lags far behind.In this paper,we analyzed the effects of high pH on Arabidopsis during seed germination and seedling development,especially root elongation.The results showed that mild alkali stress(pH 6.0~6.5) promoted plant growth,while severe high pH inhibited both seed germination,photosynthesis and root elongation.Auxin content markedly decreased at high pH,suggesting an important role of auxin in plant response to alkali stress.