|
|||||
|
|
| 海蜇毒素的提取及活性测定 | |
| 引用本文： | 蔡学新,苏秀榕,杨春,李晔.海蜇毒素的提取及活性测定[J].水产科学,2008,27(3):145-147. |
| 作者姓名： | 蔡学新 苏秀榕 杨春 李晔 |
| 作者单位： | 1. 大连市海洋与渔业局,辽宁,大连,116011 2. 宁波大学生命科学与生物工程学院,浙江,宁波,315211 3. 宁波效实中学,浙江,宁波,315012 |
| 基金项目： | 浙江省自然科学基金资助项目(Y304358)，宁波市科技局重点资助项目(00120041) |
| 摘 要： | 利用液氮速冻、石英砂研磨破碎刺胞,用水溶液、醇溶液提取海蜇和沙蜇刺胞毒素。用Sepha-dex G-75柱层析分离得到2个峰,收集2个峰的液体冷冻干燥得到毒素。利用血平板快速检测毒素的活性。沙蜇毒素的溶血活性明显高于海蜇毒素。提取物中的活性成分的分子量约为10 000~60 000。这类毒素具有溶血、心脏毒性、神经毒性、肌肉毒性、皮肤坏死,影响离子转运以及胆碱酯酶活性等作用。 |
| 关 键 词： | 海蜇 沙蜇 刺胞毒素 溶血活性 |
| 文章编号： | 1003-1111(2008)03-0145-03 |
| 修稿时间： | 2006年11月10 |
Activity Detection and Extraction of Jellyfish Venom |
|
| CAI Xue-xin,SU Xiu-rong,YANG Chun,LI Ye.Activity Detection and Extraction of Jellyfish Venom[J].Fisheries Science,2008,27(3):145-147. | |
| Authors: | CAI Xue-xin SU Xiu-rong YANG Chun LI Ye |
| Abstract: | The sting cells in jellyfish Rhopilema esculentum Kishinouye and Nemopilema nomurai were frozen rapidly in liquid nitrogen and equally volume OI cold saline and homogenized with quartz sand to extract the active components in the nematocysts.Then the nematocyst suspension was purified by column chromatography,and the activity of the venom was quickly detected by hemolytic reaction.The venom as active components in jellyfish showed both vasoconstrictor and cardiodepressive effects in rats and had molecular weight of 10 kDa~60 kDa.It was found that the venom extracted from the nematocysts had potem vasoconstrictor and cardiodepressive actions in anaesthetized rats,and the lethal effect was thermolabile and dependent on L-type calcium channels. |
| Keywords: | Rhopilema esculentum Nemopilema nomurai jellyfish venom hemolytic activity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |