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马铃薯试管苗低成本快繁方式研究
引用本文:董淑英,李梅,孙静,赵翔宇,陈振德,于秋华.马铃薯试管苗低成本快繁方式研究[J].中国马铃薯,2002(1):7-10.
作者姓名:董淑英  李梅  孙静  赵翔宇  陈振德  于秋华
作者单位:1. 青岛市农业科学研究所,青岛,266100
2. 山东农业大学,泰安,271018
摘    要:为适应脱毒种薯工厂化生产的要求 ,降低成本 ,对马铃薯脱毒试管苗快繁方式进行了研究 ,以不影响脱毒苗生长为前提 ,筛选出成本低、生长好的培养方法。研究结果表明 :①液体培养由于省去了价格昂贵的琼脂 ,培养成本降低了 80 0 3% ,并且试管苗的生长比固体培养要好 ;②在液体培养过程中 ,不加有机成分 ,培养成本降低 2 3 71% ,脱毒试管苗生长正常 ;③液体培养基的用量要少于固体培养基的用量 ,为固体培养基 1/ 2~ 1/ 3,一般以 10ml为宜

关 键 词:马铃薯  脱毒试管苗  培养基  快繁
文章编号:1001-0092(2002)01-007-03

THE STUDY ON REDUCING THE MICRO-PROPAGATION COST OF PLANTLETS
DONG Shu ying ,LI Mei ,SUN Jing ,ZHAO Xiang yu ,CHEN Zhen de ,YU Qiu hua.THE STUDY ON REDUCING THE MICRO-PROPAGATION COST OF PLANTLETS[J].Chinese Potato,2002(1):7-10.
Authors:DONG Shu ying  LI Mei  SUN Jing  ZHAO Xiang yu  CHEN Zhen de  YU Qiu hua
Institution:DONG Shu ying 1,LI Mei 1,SUN Jing 1,ZHAO Xiang yu 2,CHEN Zhen de 1,YU Qiu hua 1
Abstract:In order to meet the demand of factory production of virus free mini tuber and reduce the cost,propagation pattern of the potato virus free tuber plant was studied.Under the premise of non affecting the growth of virusfree tuber plant,we select out the propagation pattern with the lower cost and better reproduction.The result shows:1.Without the expensive agar,using liquid culture can reduce the cost by 80 03% and gain better growth than sold culture.2.In the liquid culture,if we eliminated the organic composition,we can reduce the cost by 23 71%,but get the normal growth.3.The volume of liquid culture medium is less than the solid culture medium and only 1/2~1/3 of the latter,that is,10 ml is better.$$$$
Keywords:potato  virus  free  tuber  plant  culture medium  cost  micro  propagation
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