| RT—PCR检测南方菜豆花叶病毒 |
| |
| 引用本文： | 李尉民 Hull.,R.RT—PCR检测南方菜豆花叶病毒[J].中国进出境动植检,1997(1):28-30. |
| |
| 作者姓名： | 李尉民 Hull. R |
| |
| 作者单位： | 农业部植物检疫实验所，福建农业大学 |
| |
| 摘 要： | 根据南方菜豆花叶病毒（ＳＢＭＶ）两株系Ｂ和Ｃ的核酸序列设计两对引物，利用ＲＴ－ＰＣＲ可以特异地区分纯化的和０．２ｇ病叶中的两株系，ＲＴ－ＰＣＲ检测ＳＢＭＶ－Ｂ的灵敏度为１０ｐｇ。
|
| 关 键 词： | 南方菜豆花叶病毒 检测 RT－PCR |
DETECTION OF SOUTHERN BEAN MOSAIC VIRUS(SBMV)BY RT-PCR |
| |
| Authors: | Li Weimin Roger Hull Zhang Chengliang Xie Lianhui |
| |
| Institution: | Li Weimin1 Roger Hull2 Zhang Chengliang1 Xie Lianhui3 1. |
| |
| Abstract: | Acording to the nucleotide sequences of Southern Bean Mosaic Virus(SBMV),two pairs of primers were designed.Strains B and C of SBMV extracted from 0.2g infected leaves could be differetiated by RT-PCR.The sensitivity of RT-PCR for detecting SBMV-B Virus was 10pg. |
| |
| Keywords: | Southern Bean Mosaic Virus Detection RT-PCR |
| 本文献已被 CNKI 维普 等数据库收录！ |
