CRISPR/Cas9-Targeted Knockout of Rice Susceptibility Genes OsDjA2 and OsERF104 Reveals Alternative Sources of Resistance to Pyricularia oryzae |
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Authors: | Fabiano T P K T vora Anne C cile Meunier Aurore Vernet Murielle Portefaix Joë lle Milazzo Henri Adreit Didier Tharreau Oct vio L Franco Angela Mehta |
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Institution: | 1. Department of Genetics and Biotechnology, Federal University of Juiz de Fora, Juiz de Fora-MG 36036330, Brazil;2. Embrapa Genetic Resources and Biotechnology, Brasília-DF 70770-917, Brazil;3. Genetic Improvement and Adaptation of Mediterranean and Tropical Plants, Agricultural Research Center for International Development (CIRAD), Montpellier 34398 CEDEX 5, France;4. CIRAD-INRAe-Agro Institute, University of Montpellier, Montpellier 34000, France;5. Plant Health Institute of Montpellier, CIRAD, Montpellier 34398, France;6. University of Montpellier, National Institute of Agronomic Research, Research Institute for Development, Montpellier SupAgro, Montpellier 34398, France;7. Department of Genomics Science and Biotechnology, Proteomics Analysis Center, Catholic University of Brasília, Brasília-DF 71966-900, Brazil;8. S-Inova Biotech, Catholic University of Dom Bosco, Campo Grande-MS 79117-900, Brazil |
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Abstract: | Rice genes OsDjA2 and OsERF104, encoding a chaperone protein and an APETELA2/ ethylene-responsive factor, respectively, are strongly induced in a compatible interaction with blast fungus, and also have function in plant susceptibility validated through gene silencing. Here, we reported the CRISPR/Cas9 knockout of OsDjA2 and OsERF104 genes resulting in considerable improvement of blast resistance. A total of 15 OsDjA2 (62.5%) and 17 OsERF104 (70.8%) T0 transformed lines were identified from 24 regenerated plants for each target and used in downstream experiments. Phenotyping of homozygous T1 mutant lines revealed not only a significant decrease in the number of blast lesions but also a reduction in the percentage of diseased leaf area, compared with the infected control plants. Our results supported CRISPR/Cas9-mediated target mutation in rice susceptibility genes as a potential and alternative breeding strategy for building resistance to blast disease. |
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Keywords: | gene editing plant-pathogen interaction plant immunity blast resistance rice |
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