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Rooting Characteristics of Solanum chacoense and Solanum tuberosum in Vitro
Authors:Christian T. Christensen  Lincoln Zotarelli  Kathleen G. Haynes  James Colee
Affiliation:1.Department of Horticultural Sciences,University of Florida,Gainesville,USA;2.USDA-ARS, Genetic Improvement of Fruits and Vegetables Laboratory,Beltsville,USA;3.IFAS Statistical Consulting Unit,University of Florida,Gainesville,USA
Abstract:The precise level of environmental control in vitro may aid in identifying genetically superior plant germplasm for rooting characteristics (RC) linked to increased foraging for plant nitrogen (N). The objectives of this research were to determine the phenotypic variation in root morphological responses of 49 Solanum chacoense (chc), 30 Solanum tuberosum Group Phureja – Solanum tuberosum Group Stenotomum (phu-stn), and three Solanum tuberosum (tbr) genotypes to 1.0 and 0.5 N rate in vitro for 28 d, and identify genotypes with superior RC. The 0.5 N significantly increased density of root length, surface area, and tips. All RC were significantly greater in chc than in either phu-stn or tbr. Based upon clustering on root length, surface area, and volume, the cluster with the greatest rooting values consisted of eight chc genotypes that may be utilized to initiate a breeding program to improve RC in potato.
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