Mitochondrial DNA analysis reveals cytoplasmic variation within a single cultivar of perennial ryegrass (Lolium perenne L.) |
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| Authors: | M. Sato M. Yamashita S. Kato T. Mikami Y. Shimamoto |
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| Affiliation: | (1) Laboratory of Plant Genetics and Evolution, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan;(2) Laboratory of Genetic Engineering, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan;(3) Present address: Department of Biological Science, Faculty of Agriculture, Shizuoka University, Shizuoka 422, Japan |
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| Abstract: | Summary The extent of mitochondrial DNA restriction fragment length polymorphisms among individual plant samples of perennial ryegrass was determined. A total of 72 plants from three cultivars Yorktown II, S23 and Riikka were surveyed using three restriction enzymes (BamHI,EcoRI andHindIII) and three mitochondrial gene probes (coxI, coxIII andnad9). Polymorphisms were noted within each of the two cultivars Yorktown II and S23, whereas in  Riikka no variation was detected. It seems most likely that the mitochondrial genome diversity within the same cultivar has resulted from non-homogeneous ancestor cytoplasms. The hybridization-based assay employed is simple to perform, gives unambiguous results, and may thus be used in mass screening of perennial ryegrass populations for breeding purpose. |
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| Keywords: | mitochondrial genome diversity perennial ryegrass Lolium perenne RFLP |
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