| 新疆陆地棉转木霉几丁质酶基因棉花的初步筛选 |
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| 引用本文： | 李莉,曲延英,陈全家,夏瑜华,兰杰,尤春源.新疆陆地棉转木霉几丁质酶基因棉花的初步筛选[J].新疆农业科学,2005,42(3):178-180. |
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| 作者姓名： | 李莉 曲延英 陈全家 夏瑜华 兰杰 尤春源 |
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| 作者单位： | 新疆农业大学农学院,新疆乌鲁木齐,830052 |
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| 基金项目： | 自治区"十五"科技攻关项目"棉花新品种选育及产业化"(200131101) |
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| 摘 要： | 利用花粉管通道法将构建在植物表达载体pART27上的抗黄萎病基因木霉几丁质酶(Trichodermachi-tinase)基因,导入新疆4个陆地棉品种(系)中,经标记基因阳性试验和病圃抗病性检测,获得T2代具有较高的抗病性植株30株。
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| 关 键 词： | 陆地棉 转基因 花粉管通道法 选择标记 |
| 文章编号： | 1001-4330(2005)03-0178-03 |
| 修稿时间： | 2005年3月18日 |
Preliminary selection for cotton cultivar transformed with Trichoderma chitinase gene in Xinjiang upland cotton |
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| LI Li,QU Yan-ying,Chen Quan-jia,XIA Yu-hua,LAN Jie,YOU Chun-yuan.Preliminary selection for cotton cultivar transformed with Trichoderma chitinase gene in Xinjiang upland cotton[J].Xinjiang Agricultural Sciences,2005,42(3):178-180. |
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| Authors: | LI Li QU Yan-ying Chen Quan-jia XIA Yu-hua LAN Jie YOU Chun-yuan |
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| Abstract: | Trichoderma chitinase gene in plant expression vector, pART27, was transformed into 4 cultivars(lines) of Xinjiang upland cotton by pollen-tube pathway method. 30 Trichoderma chitinase gene-transgenic in T_2 plants with satisfactory resistance to verticillum wilt was obtained through selectable marker gene identified as positive strain and cotton-resistant check. |
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| Keywords: | upland cotton transfering gene pollen tube pathway selection marker |
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