| 油茶种质资源RAPD分析I.DNA提取和PCR扩增条件建立 |
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| 引用本文： | 黄永芳,陈锡沐,雷治国,陈永忠,彭邵峰. 油茶种质资源RAPD分析I.DNA提取和PCR扩增条件建立[J]. 河南农业科学, 2004, 0(12): 22-25 |
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| 作者姓名： | 黄永芳 陈锡沐 雷治国 陈永忠 彭邵峰 |
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| 作者单位： | 1. 华南农业大学林学院,广东,广州,510642
2. 湖南省林业科学院 |
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| 基金项目： | 广东省科技攻关资助项目 (2 0 0 2C2 0 10 6 0 3)，广东省林业科技攻关项目资助 (2 0 0 2 - 13) |
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| 摘 要： | 通过比较SDS和CTAB 2种方法 ,CTAB法更适于油茶DNA的提取 ,并在CTAB法提取过程中将一些有效的去多糖、多酚的步骤进行组合 ,建立适合油茶DNA的提取方法 ,同时构建了油茶DNA的PCR扩增程序 ,并从预变性温度、Mg2 +浓度、dNTPs、TaqDNA聚合酶、模板DNA等方面确定了PCR扩增条件
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| 关 键 词： | 油茶 DNA提取 PCR |
| 文章编号： | 1004-3268(2004)12-0022-04 |
| 修稿时间： | 2004-06-04 |
Analysis of Camellia oleifera Germplasm Resources by RAPD I.DNA Extracting and Establishment of PCR Amplified Condition |
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| HUANG Yong-fang ,CHEN Xi-mu ,LEI Zhi-guo ,CHEN Yong-zhong ,PENG Shao-feng. Analysis of Camellia oleifera Germplasm Resources by RAPD I.DNA Extracting and Establishment of PCR Amplified Condition[J]. Journal of Henan Agricultural Sciences, 2004, 0(12): 22-25 |
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| Authors: | HUANG Yong-fang CHEN Xi-mu LEI Zhi-guo CHEN Yong-zhong PENG Shao-feng |
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| Affiliation: | HUANG Yong-fang 1,CHEN Xi-mu 1,LEI Zhi-guo 1,CHEN Yong-zhong 2,PENG Shao-feng 2 |
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| Abstract: | Two methods(SDS method and CTAB method) for DNA extrcating were compared.The CTAB method was the better for Camellia oleifera DNA extracting.Some process such as eliminating amylose and eliminating polyphenols were united and were ameliorated,then the suited method for Camellia oleifera DNA extracting was defined.PCR amplified procedure was established and PCR amplified conditions were defined on the factors of pre-denaturating temperature,Mg 2+ density,dNTPs density,Taq DNA polymerase and DNA density. |
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| Keywords: | Camellia oleifera DNA extracting PCR |
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