急性冷应激对金定鸭开产前内分泌活动的影响

选42羽产前的金定蛋鸭进行笼养,研究其在舍温从正常的15℃急速降到-15℃,0.5h后再恢复正常温度的12h内某些内分泌活动的规律。试验结果如下,血清中三碘甲腺原氨酸(T3)呈现抛物线变化,在应激后0.5￣1h内出现峰值(1.28±0.03ng·mL-1),然后开始下降,在应激后12h达到(1.22±0.03)ng·mL-1的最低值,其差异显著(P<0.05);甲状腺素(T4)的含量均在应激后1h出现高峰值(5.24±5.07ng·mL-1),然后迅速下降,在应激后12h达到(1.81±1.98)ng·mL-1的最低值,其差异极显著(P<0.01);皮质醇(Cort)的含量前期上升迅速,后期变化规律不明显,并在试验期间内出现了2次峰值,分别是应激后立刻出现的(1.42±0.19ng·mL-1)和在应激后12h出现的(1.38±0.21ng·mL-1),同时在应激后2h有一个小的波峰(1.37±0.07ng·mL-1),其差异不显著(P>0.05);胰岛素(Ins)的含量先下降后上升,在应激开始时是(1.15±0.01)μIU·mL-1,在应激12h后为(1.15±0.02)μIU·mL-1,其差异不显著(P>0.05);血清中的总抗氧化能力(T-AOC)在应激后1h达到高峰(32.56±3.65)IU·mL-1,然后开始下降,到应激后12h为(24.04±4.40)IU·mL-1;其差异极显著(P<0.01);丙二醛(MDA)的含量变化较大,应激后立刻降到(1.43±0.33)nmoL·mL-1,应激后12h为(1.26±0.35)nmoL·mL-1,中间不断呈现波动变化,在应激后2h达到了峰值(2.18±0.36)nmoL·mL-1,其差异显著(P<0.05)。综合本试验结果得出,在急性应激条件下,血清中的个别激素、总抗氧化能力和丙二醛可以作为应激的敏感指标。

Study on the effect of acute cold stress on incretion activity in pre-egged Jin-ding ducks in cage

The experiment was conducted to study the change rule of T3 ,T4, Cor t, Ins, MDA and T-AOC of the pre-egged Jin-ding ducks in serum during twelve hou rs when the environment temperature felt from 15 ℃ to-15 ℃ and then back. 42 ( 2 weeks before laying) Jin-ding pre-egged female ducks were randomly assigned to seven groups to do cold stress experiment. The results showed that, there was s ignificant different effect on T3 in the serum after stressed(P < 0.05), while g reat influence on T4, which significantly ascended after stressed in an hour and then descended slightly(P < 0.01); the content of cort ascended rapidly in the prophase and descended unclearly in the anaphase, there are two apices during th e twelve hours after cold stress and there was insignificant difference(P > 0.05 ); the content of Ins ascended firstly and then descended and there was signific ant inflence(P < 0.05); the T-AOC achieved apex in an hour and then descend, and there was great influence on it during twelve hours after stressed(P < 0.01); b ut there was significant effect on MDA in the serum, it descended firstly and as cended rapidly and then fluctuated with time(P < 0.05=.