| 建兰花叶病毒及齿兰环斑病毒的检测方法(综述) |
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| 引用本文： | 刘沛然,王四清. 建兰花叶病毒及齿兰环斑病毒的检测方法(综述)[J]. 热带作物学报, 2009, 30(1): 99-103 |
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| 作者姓名： | 刘沛然 王四清 |
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| 作者单位： | 北京林业大学园林学院,北京,100083;北京林业大学园林学院,北京,100083 |
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| 基金项目： | 北京市教育委员会共建计划产学研合作项目 |
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| 摘 要： | 介绍目前已广泛应用于建兰花叶病毒(Cymbidium mosaic virus,CymMV)和齿兰环斑病毒(Odontoglossum ringspot virus,ORSV)检测的电镜技术、血清学方法和分子生物学方法,以及以这3种方法为基础建立起的酶联免疫吸附反应法(enzyme linked immunosorbent assay,ELISA)、免疫胶体金技术、免疫毛细区带电泳(Inununo-capillary ZOlle electrophoresis,I-CZE)、反转录聚合酶链式反应法(Reverse transcription-polymerase chain reaction RT-PCR)、免疫捕捉PCR技术(IC-RT-PCR)、TaqMan实时RT-PCR技术.同时介绍石英晶体微天平生物传感器(Quartz Crystal Microbalance biosensor, QCM biosensor)应用检测技术、液相色谱-质谱分析和激光解吸-离子质谱分析法(LC-and MALDI-mass spectrometry)2种新检测技术.并简述了各种检测方法的优缺点及应用范围.
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| 关 键 词： | 建兰花叶病毒 齿兰环斑病毒 检测 植物病毒 |
Advances on Detection Methods of Cymbidium mosaic Virus and Odontoglossum ringspot Virus |
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| Liu Peiran and Wang Siqing. Advances on Detection Methods of Cymbidium mosaic Virus and Odontoglossum ringspot Virus[J]. Chinese Journal of Tropical Crops, 2009, 30(1): 99-103 |
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| Authors: | Liu Peiran and Wang Siqing |
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| Affiliation: | Liu Peiran,Wang Siqing College of L,scape Architecture,Beijing Forestry University,Beijing 100083 |
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| Abstract: | This paper reviews the microscopical,serological and molecular means for detecting of both CymMV and ORSV.The technologies of ELISA,Immunogold-label assay,I-CZE,RT-PCR,IC-RT-PCR and TaqMan Real-Time RT-PCR established based on the microscopical,serologival and molecular means,and two other new detection methods: QCM immunosensors and LC-and MALDI-mass spectrometry are summarized.The advantage and disadvantage in application of these detection methods are discussed. |
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| Keywords: | Cymbidium mosaic virus (CymMV) Odontoglossum tingspot virus (ORSV) detection plant virus |
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