新疆彩色棉23个品种指纹图谱的构建及遗传多样性分析

以新疆截止2012年审定的23份新彩棉品种为材料,利用SSR标记进行DNA指纹图谱的构建和遗传多样性分析。从5000对SSR引物中,挑选出多态性高、稳定性好、均匀分布在棉花26条染色体上的52对引物,在23份新彩棉品种中筛选出核心引物47对,SSR扩增检测到多态性基因型位点数共计162个,每个标记检测到的基因型位点数在2~7之间,平均为3.45个;引物多态信息量(PIC)值介于0.4537~0.8686之间,平均值为0.7096。结果显示:在23份新彩棉品种中,14份品种采用特异或特征引物可以一次性区分开,其余9份品种需要采用引物组合来实现区别该品种与其他品种。最少选用18对特异引物及组合引物就可以完全区分开新彩棉1~23号品种。利用18对SSR标记构建了新彩棉1号至23号品种的指纹图谱。利用NTSYS-pcV2.10软件聚类分析表明:23个新彩棉品种遗传相似系数变化范围是0.3781~0.9298,平均为0.5511,表明新彩棉品种之间存在着丰富的遗传多样性。

Construction of DNA Fingerprinting and Analysis of Genetic Diversity with SSR Markers for 23 color Cotton Cultivars in Xinjiang

Twenty-three Xincaimian cotton cultivars as authorized until 2012 were used as materials in the construction of DNA fingerprinting and analysis of genetic diversity with simple sequence repeat(SSR) markers. Fifty-two primer pairs with high polymorphism, good repeatability, and even distribution on the 26 chromosomes were screened out from 5000 pairs of SSR primers to genotype the 23 cultivars. A total of 47 SSR primer pairs were polymorphic and generated 162 polymorphic loci among the 23 cultivars; each marker detected 2-7 polymorphic loci with an average of 3.45; the polymorphism information content ranged from 0.4537 to 0.8686, with an average of 0.7096. The results showed that there 14 cultivars could be differentiated by specific or characteristic primer, the rest 9 cultivars could be identified by primer combinations. In the end, 18 specific or characteristic and primer combinations could completely differentiate the 23 Xincaimian cotton cultivars from each other. DNA fingerprinting of Xincaimian 1 to Xincaimian 23 were constructed with 18 pairs of SSR markers. NTSYSpc v2.10 software was used to analyze genetic diversity of the 23 cultivars; the results indicated that genetic similarity coefficient of the 23 cultivars ranged from 0.3781 to 0.9298 with an average of 0.5511, which showed that there was abundant genetic diversity in Xincaimian cotton varieties.