陆地棉繁殖群体大小对遗传完整性的影响

利用形态标记和SSR分子标记技术分析了澳L23/757(鸡脚叶)、中棉所19(正常叶)、红桃(红叶)的25、50、100、150株不同自然授粉繁殖群体的遗传完整性。结果表明,3个种质间的形态标记的天然异交率和SSR标记遗传纯度有显著差异,但4个群体大小间的天然异交率和SSR标记遗传纯度的差异均不显著。说明棉花繁殖群体为25株时,可作为棉花种质繁殖更新过程中的最低繁殖群体量,能较好地保持繁殖种质的遗传完整性。

Effects of Population Size on Seed Increase for Genetic Integrity of Cotton (Gossypium hirsutum L.)

The genetic integrity of different natural pollination populations with 25, 50, 100, and 150 plants in three germplasms of AoL23/757 (okra-leaf), CCRI 19 (normal-leaf) and Hongtao (red-leaf) was analyzed using morphological characteristics and SSR markers. The results showed that both the natural outcrossing rates of morphological markers and the molecular genetic purity of SSR markers among the three germplasms were significantly different, but the four breeding population sizes did not display significant differences. This proved that 25 plants could be used as the lowest regeneration population size, for efficient maintenance of the genetic integrity of cotton germplasms (Gossypium hirsutum L.).