| EMS诱导巴西橡胶树花药次生体细胞胚突变方法初探 |
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| 引用本文： | 陈祖兴,赵建文,翟琪麟,李维国.EMS诱导巴西橡胶树花药次生体细胞胚突变方法初探[J].热带农业科学,2012,32(2):32-35. |
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| 作者姓名： | 陈祖兴 赵建文 翟琪麟 李维国 |
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| 作者单位： | 1. 海南大学农学院,海南儋州571737／中国热带农业科学院橡胶研究所／国家橡胶树育种中心,海南儋州571737
2. 中国热带农业科学院橡胶研究所／国家橡胶树育种中心,海南儋州,571737 |
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| 摘 要： | 以巴西橡胶树热研7-33-97未成熟花药次生体细胞胚为材料,采用化学诱变剂EMS(甲基磺酸乙酯)诱变处理,设置4个浓度(0%、0.1%、0.3%、0.5%)、4个处理时间(2、4、6、8 h)共16个处理组合,以"半致死剂量"为选择标准,确定了巴西橡胶树热研7-33-97未成熟花药次生体细胞胚的适应诱导组合为EMS浓度0.5%,处理时间4 h。
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| 关 键 词： | 次生胚 EMS 化学诱变 |
Mutation Method for Rubber Secondary Embryo Induced by EMS |
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| CHEN Zuxing,ZHAO Jianwen,ZHAI Qilin,LI Weiguo.Mutation Method for Rubber Secondary Embryo Induced by EMS[J].Chinese Journal of Tropical Agriculture,2012,32(2):32-35. |
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| Authors: | CHEN Zuxing ZHAO Jianwen ZHAI Qilin LI Weiguo |
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| Institution: | 1 College of Agronomy,Hainan University,Danzhou,Hainan 571737; 2 State Center for Rubber Breeding/Rubber Institute,CATAS,Danzhou,Hainan 571737) |
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| Abstract: | Chemical mutagenesis can create a variety of mutants effectively.Mutagenic efficiency can be improved if the appropriate conditions for mutagenesis are determined.In this paper,we employed the immature secondary somatic embryos of anther from the Brazilian rubber tree Reyan 7-33-97 as material,and then we set four treatment levels,containing 0%,0.1%,0.3% and 0.5%,respectively,and four treatment time,which was 2 h,4 h,6 h and 8 h,respectively,a total of 16 combinations treated with chemical mutagen EMS.The results showed that it could reach the "half lethal dose" effect when the treatment time was 4 h and the concentration was 0.5%. |
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| Keywords: | secondary embryo EMS chemical mutagenesis |
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