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亚精胺浸种对渗透胁迫下玉米种子萌发和淀粉酶活性的影响
引用本文:杜红阳,侯小歌,刘怀攀.亚精胺浸种对渗透胁迫下玉米种子萌发和淀粉酶活性的影响[J].河南农业科学,2010(5).
作者姓名:杜红阳  侯小歌  刘怀攀
作者单位:1. 周口师范学院,生命科学系,河南,周口,466000
2. 周口师范学院,生命科学系,河南,周口,466000;河南农业大学,作物生长与发育调控省重点实验室,河南,郑州,450002
基金项目:国家自然科学基金,河南省基础与前沿技术研究计划项目,河南省教育厅自然科学基础研究计划 
摘    要:研究了亚精胺浸种对渗透胁迫下玉米(Zea mays L.)种子萌发和淀粉酶活性的影响。结果表明,与对照相比,在渗透胁迫下,玉米发芽势、发芽率和胚芽鞘长度均下降,且萌发过程中的淀粉酶活性下降,淀粉含量上升,可溶性糖、还原糖含量下降;用亚精胺(Spd)浸种处理后,抑制了玉米种子在渗透胁迫条件下其发芽势、发芽率、胚芽鞘长度、淀粉酶活性、可溶性糖和还原糖含量下降以及淀粉含量的上升。上述结果表明,Spd浸种处理通过提高渗透胁迫下玉米种子萌发过程中的淀粉酶的活性,促进淀粉的降解,从而促进渗透胁迫下玉米种子的萌发。

关 键 词:亚精胺浸种  玉米  萌发  淀粉酶

Effect of Seed Soaking with Spermidine on Seed Germination and Amylase Activity of Maize under Osmotic Stress
DU Hong-yang,HOU Xiao-ge,LIU Huai-pan.Effect of Seed Soaking with Spermidine on Seed Germination and Amylase Activity of Maize under Osmotic Stress[J].Journal of Henan Agricultural Sciences,2010(5).
Authors:DU Hong-yang  HOU Xiao-ge  LIU Huai-pan
Abstract:The effects of seed soaking with spermidine on seed germination and amylase activity of maize were investigated under osmotic stress.The results showed that the osmotic stress decreased the germination rate,germination energy,coleoptile length,amylase activity and the contents of reduced and soluble sugers,and increased the starch content,while seed soaking with spermidine could alleviate the inhibiting effects of osmotic stress.This suggested that Spd seed-soaking could facilitate maize seed germination by enhancing the amylase activity and promoting the starch hydrolysis in seed under osmotic stress.
Keywords:Spermidine seed-soaking  Maize(Zea mays L  )  Germination  Amylase
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