不同中药对奶牛乳房炎病原菌抑菌效果的观察

本试验采用琼脂平板扩散法(打孔法)，研究了黄芩等21种中药对从乳房炎奶牛乳汁中分离出的3种主要致病菌的体外抑菌情况。结果发现，大肠杆菌对黄芩高度敏感，对丹参、青翘属中度敏感；金黄色葡萄球菌对黄芩、青翘、丹参、地榆、红花、蒲公英高度敏感，对枳壳、忍冬藤、萹蓄、苦地丁、蟾酥、木通中度敏感；无乳链球对地榆、当归属高度敏感，对青翘、黄芩、蒲公英、丹参、苦地丁、红花、川芎、枳壳中度敏感。为防治奶牛乳房炎有效中药复方的筛选提供了理论依据。

Observation of the Vitro bacteriostasis Effects of Chinese Herbal Medicine on the Major Pathogenic Bacteria Causing Mastitis

Three kinds of major pathogenic microorganisms that cause mastitis of diary cows were selected and the in vitro antibiosis effects of 21 kinds of Chinese medicinal herbs such as Radix Scutellariae et.al. were tested through the method of stiletto. Twenty four nutrient agar medium were divided into there groups. Smear Escherichia Coli on A1-A8 nutrient agar medium homogeneous. Smear S. aurous on B1-B8 nutrient agar medium homogeneous. Smear Streptococcus agalactiae on C1-C8 nutrient agar medium homogeneous. Then slotting uniform upon the nutrient agar medium, respectively, each plate six hole. Add 20μL of every medical solution into each hole, and two holes were made to parallel samples. Add 21 chinese medicine to A1-A7，B1-B7，C1-C7, respectively,and A8,B8,C8 were the normal control which were added deionized water only. These nutrient agar medium were incubated at 37℃ for 24 h. Bacterial existence and antibacterial circle diameter were observed. We found that different medicine for different strains produce different effects.The experiment results showed above supplied the scientific evidence for filtering Chinese medicinal herbs to prevent and cure the mastitis.