| 正交试验优化银杏胚愈伤组织继代培养及黄酮积累 |
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| 引用本文： | 胡燕梅,张治华,周骏江,梅双喜,宋鹏飞.正交试验优化银杏胚愈伤组织继代培养及黄酮积累[J].北方园艺,2009(12). |
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| 作者姓名： | 胡燕梅 张治华 周骏江 梅双喜 宋鹏飞 |
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| 作者单位： | 武汉生物工程学院,生物工程系,湖北,武汉,430415 |
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| 基金项目： | 湖北省教育厅科学技术研究资助项目，武汉市高校科学研究资助项目 |
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| 摘 要： | 采用正交试验研究了基本培养基、NAA、6-BA、植酸(PA)4因素各3水平对银杏胚愈伤组织继代培养及黄酮积累的影响,并测定了愈伤组织生长曲线.结果表明:银杏胚愈伤组织继代培养的最佳组合是N6+NAA 3.0 mg/L+6-BA 2.0 mg/L+0.1%PA,各因子影响程度为基本培养基＞NAA＞6-BA＞PA;愈伤组织黄酮积累量最佳组合是B5+NAA 0.1 mg/L+6-BA 0.5mg/L+0.05%PA,各因子影响程度为NAA＞PA＞6-BA＞基本培养基.愈伤组织生长曲线基本呈"S"型,黄酮积累的适宜培养周期为40～50 d.
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| 关 键 词： | 银杏 正交试验 愈伤组织 黄酮 植酸 |
Optimizes the Callus Growth and Flavonoids Formation in Embryo of Ginkgo biloba L. by Orthogonal Test |
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| HU Yan-mei,ZHANG Zhi-hua,ZHOU Jun-jiang,MEI Shuang-xi,SONG Peng-fei.Optimizes the Callus Growth and Flavonoids Formation in Embryo of Ginkgo biloba L. by Orthogonal Test[J].Northern Horticulture,2009(12). |
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| Authors: | HU Yan-mei ZHANG Zhi-hua ZHOU Jun-jiang MEI Shuang-xi SONG Peng-fei |
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| Institution: | HU Yan-mei,ZHANG Zhi-hua,ZHOU Jun-jiang,MEI Shuang-xi,SONG Peng-fei(Department of Bioengineering,Wuhan Bioengineering Institute,Wuhan,Hubei 430415,China) |
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| Abstract: | The effects of different concentration and combinatibns of NAA,6-BA,PA and minimal medium on embryo a callus of Ginkgo biloba L.for subculture and flavonoids formation were studied by using orthogonal test,and combinations of optimum treatment were selected.The results showed:the optimum combinations were N6+NAA 3.0 mg/L+6-BA 2.0 mg/L+0.1%PA for embryo callus subculture and effect sequence of four effects was minimal medium>NAA>6-BA>PA;the optimum combinations were B5+NAA 0.1mg/L+6-BA 0.5 mg/L+0.05%PA for f... |
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| Keywords: | Ginkgo biloba L Orthogonal test Callus Flavonoids |
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