| 洋葱SRAP- PCR体系的优化研究 |
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| 引用本文： | 霍雨猛,缪军,杨妍妍,刘冰江,张一卉,吴雄,何启伟.洋葱SRAP- PCR体系的优化研究[J].山东农业科学,2011(4):9-11. |
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| 作者姓名： | 霍雨猛 缪军 杨妍妍 刘冰江 张一卉 吴雄 何启伟 |
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| 作者单位： | 山东省农业科学院蔬菜研究所/山东省设施蔬菜生物学重点实验室/国家蔬菜改良中心山东分中心,山东济南,250100 |
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| 基金项目： | 国家自然科学基金项目(30871706); 山东省农业良种工程项目(200903018); 山东省博士后创新基金(200903016)资助 |
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| 摘 要： | 优化了洋葱SRAP- PCR反应体系的模板浓度和Mg2+浓度.采用8对不同的引物组合和2份不同的洋葱材料进行验证实验,结果表明,在20μl的反应体系中,模板的量在180～210 ng均可获得清晰的扩增,低于180 ng,扩增带模糊,高于210 ng扩增背景严重；合适的Mg2+浓度在1.5～2.0 mmol/L之间；使用...
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| 关 键 词： | 洋葱 SRAP 分子标记 |
Optimization of SRAP-PCR System for Onion |
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| HUO Yu-meng,MIAO Jun,YANG Yan-yan,LIU Bing-jiang,ZHANG Yi-hui,WU Xiong,HE Qi-wei.Optimization of SRAP-PCR System for Onion[J].Shandong Agricultural Sciences,2011(4):9-11. |
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| Authors: | HUO Yu-meng MIAO Jun YANG Yan-yan LIU Bing-jiang ZHANG Yi-hui WU Xiong HE Qi-wei |
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| Institution: | HUO Yu-meng,MIAO Jun,YANG Yan-yan,LIU Bing-jiang,ZHANG Yi-hui,WU Xiong,HE Qi-wei(Vegetable Research Institute,Shandong Academy of Agricultural Sciences/ Key Laboratory for Biology of Greenhouse Vegetable of Shandong Province/ Shandong Branch of National Center for Vegetable Improvement,Jinan 250100,China) |
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| Abstract: | The concentrations of DNA-template and Mg2+ were optimized to establish the SRAP molecular marker system in onion.The system had been verified by eight pairs of primers and two onion materials.In 20 μl reaction system,180～210 ng DNA template and 1.5～2.0 mmol/L Mg2+ were more suitable for onion SRAP marker system.The amplified fragments obtained by this optimized system were clear,distinguishable,steady and reliable for different primer combinations and onion varieties. |
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| Keywords: | Onion SRAP Molecular marker |
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