| 不同碳氮源对复合木质素降解菌木质素降解酶系活力的影响 |
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| 引用本文： | 范寰,梁军锋,赵润,张金凤,张洪生.不同碳氮源对复合木质素降解菌木质素降解酶系活力的影响[J].天津农业科学,2010,16(2):18-20. |
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| 作者姓名： | 范寰 梁军锋 赵润 张金凤 张洪生 |
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| 作者单位： | 1. 天津市畜牧兽医研究所,天津,300112
2. 农业部环境保护科研监测所资源再生研究室,天津,300191 |
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| 基金项目： | 农业部产地环境与农产品安全重点开放实验室开放基金 |
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| 摘 要： | 通过小麦秸秆固态发酵试验,研究了不同的碳、氮源对2株侧耳属白腐真菌Tf1(Pleurotus sajor-caju)和JG1(Pleurotus Cornucopiae Roll)混合发酵产酶活力的影响。结果表明,Lip和MnP是复合木质素降解菌Tf1+JG1主要的木质素降解酶。以淀粉为碳源,麸皮为氮源时总酶活最高,分别为1419.01U/g和1626.64U/g。
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| 关 键 词： | 碳源 氮源 木质素降解菌 木质素过氧化物酶 锰过氧化物酶 漆酶 |
Effect of Different Carbon and Nitrogen Sources on Lignin Degradation Enzyme Activity of Combined Lignin-Degrading Fungus |
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| FAN Huan,LIANG Jun-feng,ZHAO Run,ZHANG Jin-feng,ZHANG Hong-sheng.Effect of Different Carbon and Nitrogen Sources on Lignin Degradation Enzyme Activity of Combined Lignin-Degrading Fungus[J].Tianjin Agricultural Sciences,2010,16(2):18-20. |
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| Authors: | FAN Huan LIANG Jun-feng ZHAO Run ZHANG Jin-feng ZHANG Hong-sheng |
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| Institution: | FAN Huan1,LIANG Jun-feng2,ZHAO Run2,ZHANG Jin-feng2,ZHANG Hong-sheng2 (1.Tianjin Institute of Animal Husb,ry , Veterinary Medicine,Tianjin 300112,China,2.Institute of Agro-environmental Protection,Ministry of Agriculture,Tianjin 300191,China) |
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| Abstract: | The present study was conducted to investigate the effect of different carbon and nitrogen sources on ligninolytic enzyme activity of the combination of Tf1(Pleurotus sajor-caju)and JG1(Pleurotus Cornucopiae Roll)of the wheat straw after the substrate solid state fermentation. Results showed that Lip and MnP were the major lignin-degrading enzymes of Tf1+JG1. Starch and wheat bran were the optimum carbon and nitrogen sources. After 9 d fermentation. the total enzyme activities were respectively 1 419.01 U/g... |
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| Keywords: | carbon sources nitrogen sources lignin-degrading fungus lignin peroxidases Mn-dependent peroxidases Laccase |
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