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Evaluation of the colonization capabilities of Salmonella Enteritidis in Quails using an RT-PCR approach
Authors:Guang-Zhi He  Yong Feng  Shu-Xuan Deng  Li-Fang He  Chuan-Wei An  Wei-Yi Tian
Institution:1. Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China;2. Agricultural Office of Dalingshan Town, Dongguan 523830, Guandong Province, China;3. Department of Parasitology, Zunyi Medical College, Zunyi 563003, Guizhou Province, China
Abstract:We used a real-time PCR assay and indirect fluorescent antibody (IFA) assay to detect genomic DNA of Salmonella Enteritidis in the internal organs of quails after an oral challenge. The results showed that S. Enteritidis was detected in all the samples at different time points. This study will assist a future understanding of the pathogenesis of S. Enteritidis.
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