| DGGE/TGGE技术在微生物生态学中的应用 |
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| 引用本文： | 陈静,马松成,毛华明.DGGE/TGGE技术在微生物生态学中的应用[J].中国畜牧兽医,2006,33(11):47-50. |
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| 作者姓名： | 陈静 马松成 毛华明 |
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| 作者单位： | 云南农业大学动物营养与饲料科学重点实验室,昆明,650201 |
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| 摘 要： | 变性梯度凝胶电泳(deaturing grad ien t ge l e lectrophores is,DGGE)和温度梯度凝胶电泳(tem perature grad ien t ge le lectrophores is,TGGE)可以直接分离PCR扩增片段,作为一种分子指纹技术而逐渐被人们应用于微生态研究中。通过DGGE/TGGE对微生物组成的遗传特性进行表征,不但省去了菌种分离耗时耗力的工作量,更可鉴定出根据传统方法无法分离出来的菌种。作者重点描述了DGGE/TGGE的基本原理以及在胃肠道微生态研究中的应用。
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| 关 键 词： | 变性梯度凝胶电泳 温度梯度凝胶电泳 胃肠道微生态 多样性 |
| 文章编号： | 1671-7236（2006）11-0047-03 |
| 收稿时间： | 2006-05-08 |
| 修稿时间： | 2006-05-08 |
Application of DGGE/TGGE in Gastrointestinal Micro-ecology |
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| CHEN Jing,MA Song-cheng,MAO Hua-ming.Application of DGGE/TGGE in Gastrointestinal Micro-ecology[J].China Animal Husbandry & Veterinary Medicine,2006,33(11):47-50. |
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| Authors: | CHEN Jing MA Song-cheng MAO Hua-ming |
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| Institution: | Key Laboratory of Animal Nutrition and Feed,Yunnan Agricultural University, Kunming 650201, China |
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| Abstract: | Denaturing or Temperature Gradient Gel Electrophoresis(DGGE/TGGE),as molecular fingerprinting techniques which possess the capability to separate polymerase chain reaction(PCR)-generated DNA products,have been successfully applied to the analysis of gastrointestinal(GI) microbial ecosystems.The feasibility of DGGE/TGGE in the investigation of complex microbial communities removes the time-and labor-consuming isolations of microbes based on the conventional methods,but also reveal the diversity of microbial communities and identify the uncultured microorganisms in the microbial ecosystems.This review summarizes the basic principle of DGGE/TGGE and their applications in the monitoring of gastrointestinal microbial ecosystems. |
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| Keywords: | DGGE TGGE GI microbial ecosystems diversity |
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