灰葡萄孢菌两种发酵粗提物的除草活性研究

测定了灰葡萄孢菌(Botrytis cinerea)固体和液体发酵粗提物对小麦、高粱、黄瓜、油菜的幼苗和幼根生长抑制活性。浓度20 mg/mL的固、液粗提物对4种供试植物的幼苗和幼根的抑制率均达到100%;对4种植物的幼苗和幼根同时具有抑制作用的最小有效浓度均为0.62 mg/mL;相同浓度的粗提物(5 mg/mL),液体发酵粗提物对小麦幼苗的抑制作用显著高于固体粗提物,而对高粱幼根和黄瓜幼苗的抑制作用却显著低于固体粗提物(p<0.05),对高粱幼苗、小麦幼根、黄瓜幼根、油菜幼苗和幼根的抑制作用与固体粗提物均无显著差异(p>0.05)。综合评价除草活性效果,灰葡萄孢菌固体和液体发酵粗提物均有很高除草活性,而固体发酵因原料廉价、操作简单具有更为广泛的应用前景。

Herbicidal Activity of Coarse Extracts form Botrytis cinerea Produced by Different Fermentation Methods

Two fermentation methods,solid fermentation and liquid fermentation,were used to get Botrytis cinerea coarse extracts.The inhibitory effect on the seedlings growth and root length of wheat,sorghum,rape and cucumber of the two coarse extracts were studied.The two coarse extracts inhibited growth of the four tested plants seedlings and roots completely at concentration of 20 mg/mL,and both of the minimum effective in hibitory concentration were 0.62 mg/mL.At the same concentration(5 mg/mL),the inhibition to wheat seeding growth of the liquid extracts was higher than the solid extract.But to cucumber seeding growth and sorghum roots growth,the inhibition was lower than the solid of(p<0.05).There were no difference between two extracts in in hibition totherootsgrowth ofwheat,cucumber,rape and theseedinggrowth of rape and sorghum(p>0.05).The results indicated the two coarse extracts of B.cinerea both have strong herbicidal activity,but the solid has wider development prospect than the liquid because of its in expensive raw materials and simple of operation.