| 用毛细管电泳检测伊氏锥虫微环DNA PCR扩增产物 |
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| 引用本文： | 王云飞,孟秀珍.用毛细管电泳检测伊氏锥虫微环DNA PCR扩增产物[J].中国兽医寄生虫病,1998,6(1):5-7. |
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| 作者姓名： | 王云飞 孟秀珍 |
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| 作者单位： | 中国农业科学院上海家畜寄生虫病研究所!200232 |
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| 摘 要： | 毛细管电泳技术用于ＤＮＡ电泳分析，具有快速，样品用量少，高重复率等特点。本文应用毛细管电泳对动基体微环ＤＮＡ ＰＣＲ扩增产物进行检测，结果表明扩增产物分子量片段为３００ｂｐ到６００ｂｐ之间，与实验设计相符，且比较纯净，可用于下一步测序分析。
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| 关 键 词： | 伊氏锥虫 PCR 毛细管电泳 检测 |
DETECT PCR PRODUCT OF MINI-CIRCLE DNA OF TRYPANOSOMA EVANSI BY CAPILLARY ELECTROPHORESIS |
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| Wang Yunfei, Meng Xiuzhen.DETECT PCR PRODUCT OF MINI-CIRCLE DNA OF TRYPANOSOMA EVANSI BY CAPILLARY ELECTROPHORESIS[J].Chinese Journal of Veterinary Parasitology,1998,6(1):5-7. |
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| Authors: | Wang Yunfei Meng Xiuzhen |
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| Abstract: | Capillary electrophoresis can be used in DNA electrophoresis analysis, which offers fast separation, needs only minute quantities of sample and gives high quantitive reproducibility. It was used to detect the PCR product of mini-circle DNA of Trypanosoma evansi. The results showed that the molecular weight of the PCR product is between 300 hp and 600 hp, as agreed with the experiment design. It was proved that the product was comparatively pure, could be used for DNA sequence analysis. |
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| Keywords: | Trypanosoma evansi PCR capillary electrophoresis |
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