| 中国白菜AFLP分子遗传图谱的构建 |
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| 引用本文： | 王美,张凤兰,孟祥栋,刘秀村,赵岫云,樊治成.中国白菜AFLP分子遗传图谱的构建[J].华北农学报,2004,19(1):28-33. |
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| 作者姓名： | 王美 张凤兰 孟祥栋 刘秀村 赵岫云 樊治成 |
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| 作者单位： | 北京市农林科学院蔬菜研究中心,北京,100089;山东农业大学,园艺学院,山东,泰安,271018;北京市农林科学院蔬菜研究中心,北京,100089;山东农业大学,园艺学院,山东,泰安,271018 |
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| 基金项目： | 北京市科技新星项目资助 |
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| 摘 要： | 以白菜高抗TuMV白心株系91 112和高感TuMV桔红心株系T12 91为亲本建立的小孢子DH系作为图谱构建群体,以AFLP标记来构建白菜连锁图谱。通过对64对AFLP引物组合的筛选,利用20对引物得到了263个AFLP多态性位点。经Mapmaker/EXP3.0软件处理,构建了1张含255个标记位点,10个连锁群,覆盖长度为883.7cM的连锁图。255个AFLP位点中偏离孟德尔遗传的比率(P<0.05)为15 0%和27 5%(P<0.01)。
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| 关 键 词： | 大白菜 双单倍体(DH) 扩增片段长度多态性(AFLP) 遗传图谱构建 |
| 文章编号： | 1000-7091(2004)01-0028-06 |
A Linkage Map Construction for Chinese Cabbage Based on AFLP Markers Using DH Population |
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| WANG Mei.A Linkage Map Construction for Chinese Cabbage Based on AFLP Markers Using DH Population[J].Acta Agriculturae Boreali-Sinica,2004,19(1):28-33. |
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| Authors: | WANG Mei |
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| Abstract: | A molecular genetic map for Chinese cabbage was constructed based on AFLPs (Amplified Fragment Length Polymorphism) markers using DNA samples extracted from a doubled haploid population obtained through microspore culture from the F_1 between two homozygous parents: 91-112 and T12-19. By screening 64 AFLP primer combinations, 263 polymorphic bands were obtained from 20 pairs of primers. These genetic markers covered 883.7 centMorgans (cM) and belonged to ten linkage groups by using Mapmaker 3.0 software analysis. Markers Deviated from Mendelian segregation ratios were observed to be 15.0% (P<0.05) and (27.5%) (P<0.01) respectively. |
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| Keywords: | Chinese cabbage AFLP Doubled haploid (DH) Genetic linkage map |
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